USP46 Antibody (CL0364) - BSA Free

Western Blot: USP46 Antibody (CL0364) [NBP2-30417] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG

Immunocytochemistry/Immunofluorescence: USP46 Antibody (CL0364) [NBP2-30417] - Staining of U-2 OS cells using the anti-USP46 monoclonal antibody, showing specific staining in nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: CL0364
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

USP46 Antibody (CL0364) - BSA Free Summary

• Description: Novus Biologicals Mouse USP46 Antibody (CL0364) - BSA Free (NBP2-30417) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P62068, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (100%), Rat (99%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP46
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for USP46 Antibody (CL0364) - BSA Free

• Deubiquitinating enzyme 46
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ11850
• FLJ12552
• FLJ14283
• FLJ39393
• ubiquitin carboxyl-terminal hydrolase 46
• ubiquitin specific peptidase 46
• ubiquitin specific protease 46
• ubiquitin thioesterase 46
• Ubiquitin thiolesterase 46
• Ubiquitin-specific-processing protease 46
• USP46

Background

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinatedaction of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteineproteases that function as deubiquitinating enzymes (Quesada et al., 2004 (PubMed 14715245)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP46
• Uniprot:
  • P62068()