UT2/SLC14A2 Antibody

Images

 
Western Blot: UT2/SLC14A2 Antibody [NBP1-44375] - analysis of Rat Inner medulla showing detection of UT-A1 protein using Rabbit Anti-UT-A1 Polyclonal Antibody. Primary Antibody: Rabbit Anti-UT-A1 Polyclonal Antibody at ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

UT2/SLC14A2 Antibody Summary

Immunogen
Produced against a synthetic peptide mapped to the C-terminal tail (amino acids 911-929) of rat UT-A1 (antibody designation L194)
Localization
Cell Membrane, Apical Cell Membrane
Specificity
Detects approx 97 and 127kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC14A2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
1 ug/ml of UT-A1 Antibody was sufficient for detection of UT-A1 in 20 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UT2/SLC14A2 Antibody

  • FLJ16167
  • HUT2UT-A2
  • hUT-A6
  • MGC119566
  • SLC14A2
  • solute carrier family 14 (urea transporter), member 2
  • Solute carrier family 14 member 2
  • urea transporter 2
  • Urea transporter, kidney
  • urea transporter-2
  • UT2
  • UT2MGC119567
  • UTA
  • UT-A2
  • UTR

Background

UT-A1, a kidney-specific urea transporter is expressed in the renal collecting duct where it mediates transepithelial urea transport and is a target for regulation by vasopressin. Urea movement out of the collecting duct in the inner medulla of the kidney allows accumulation of urea in the medullary interstitium, thereby allowing maximum water reabsorption from the collecting ducts. (The antibody also recognizes a second protein from the UT-A gene driven by transcription from an alternative promotor and expressed in the thin descending limb of Henle, viz. UT-A2.) (1, 2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC14A2
Entrez
Uniprot