USF2 Antibody (5E9) - Azide and BSA Free

Western Blot: USF2 Antibody (5E9) [H00007392-M01] - USF2 monoclonal antibody (M01), clone 5E9. Analysis of USF2 expression in different cell lines.

Immunocytochemistry/Immunofluorescence: USF2 Antibody (5E9) [H00007392-M01] - Analysis of monoclonal antibody to USF2 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: USF2 Antibody (5E9) [H00007392-M01] - Analysis of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 1 ug/ml.

Western Blot: USF2 Antibody (5E9) [H00007392-M01] - USF2 monoclonal antibody (M01), clone 5E9. Analysis of USF2 expression in PC-12.

Western Blot: USF2 Antibody (5E9) [H00007392-M01] - USF2 monoclonal antibody (M01), clone 5E9. Analysis of USF2 expression in Hela S3 NE.

Western Blot: USF2 Antibody (5E9) [H00007392-M01] - Analysis of USF2 expression in transfected 293T cell line by USF2 monoclonal antibody (M01), clone 5E9.Lane 1: USF2 transfected lysate(37 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: USF2 Antibody (5E9) [H00007392-M01] - Detection limit for recombinant GST tagged USF2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 5E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

USF2 Antibody (5E9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: USF2 (NP_003358, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRTETNGGQVTYRVVQVTDGQLDGQGDTAGAVS
• Specificity: USF2 - upstream transcription factor 2, c-fos interacting
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: USF2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USF2 Antibody (5E9) - Azide and BSA Free

• BHLHB12
• bHLHb12upstream stimulatory factor 2
• Class B basic helix-loop-helix protein 12
• FIPc-fos interacting protein
• FOS-interacting protein
• Major late transcription factor 2
• Upstream transcription factor 2
• upstream transcription factor 2, c-fos interacting

Background

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USF2 Antibody (H00007392-M01)(1)

Publication using H00007392-M01

• Beaver LM, Buchanan A, Sokolowski EI et al. Transcriptome analysis reveals a dynamic and differential transcriptional response to sulforaphane in normal and prostate cancer cells and suggests a role for Sp1 in chemoprevention. Mol Nutr Food Res 2014-07-14 [PMID: 25044704] (WB, Human)
  
  Details:
  USF2 antibody (clone 5E9) used for WB on nuclear lysates of PC-3 and LNCaP treated or not with Sulforaphane (SFN - an isothiocyanate produced by enzyme myrosinase's interaction with precursor glucoraphanin found in cruciferous vegetables like broccoli). USF2 used for normalizing the protein loading vs densitometry of nuclear Sp1 and Sp3 proteins (Figure 5B).

Bioinformatics

• Gene Symbol: USF2
• Entrez:
  • 7392(Human)
• OMIM:
  • 600390(Human)
• Uniprot:
  • NP_003358(Human)