Uromodulin Antibody [Unconjugated]

Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Uromodulin at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western shows lysates of Exosome Standards (Human Urine) (NBP2-49840) and human kidney tissue, loaded at 0.5 mg/ml. A specific band was detected for Uromodulin at approximately 149 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Uromodulin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Uromodulin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Uromodulin isoform 1
  Asp25-Ser614
  Accession # P07911
• Specificity: Detects human Uromodulin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse Uromodulin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: UMOD
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Uromodulin Antibody [Unconjugated]

• ADMCKD2
• FJHN
• HNFJ
• HNFJ1
• MCKD2
• Tamm-Horsfall glycoprotein
• Tamm-Horsfall urinary glycoprotein
• THGP
• THP
• UMOD
• uromodulin (uromucoid, Tamm-Horsfall glycoprotein)
• Uromodulin
• Uromucoid

Background

Uromodulin (also Tamm-Horsfall glycoprotein or THP) is an 85‑95 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidnay tubules. Human Uromodulin is GPI‑linked. Its proprecursor is 616 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑149), a ZP domain that mediates oligomerization (aa 334‑589) and a cleavable C‑terminal propeptide (aa 615‑640). There are multiple splice variants. One shows a deletion of aa 67‑199, a second shows a nine aa substitution for aa 609‑640, a third shows a Pro substitution for aa 205‑234 and a fourth shows a 66 aa substitution for aa 613‑640. Over aa 25‑614, human Uromodulin is 78% aa identical to mouse Uromodulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Uromodulin Antibody (AF5144)(6)

Publications using AF5144

• Kuehl, M;Okabayashi, Y;Wong, MN;Gernhold, L;Gut, G;Kaiser, N;Schwerk, M;Gräfe, SK;Ma, FY;Tanevski, J;Schäfer, PSL;Mezher, S;Sarabia Del Castillo, J;Goldbeck-Strieder, T;Zolotareva, O;Hartung, M;Delgado Chaves, FM;Klinkert, L;Gnirck, AC;Spehr, M;Fleck, D;Joodaki, M;Parra, V;Shaigan, M;Diebold, M;Prinz, M;Kranz, J;Kux, JM;Braun, F;Kretz, O;Wu, H;Grahammer, F;Heins, S;Zimmermann, M;Haas, F;Kylies, D;Wanner, N;Czogalla, J;Dumoulin, B;Zolotarev, N;Lindenmeyer, M;Karlson, P;Nyengaard, JR;Sebode, M;Weidemann, S;Wiech, T;Groene, HJ;Tomas, NM;Meyer-Schwesinger, C;Kuppe, C;Kramann, R;Karras, A;Bruneval, P;Tharaux, PL;Pastene, D;Yard, B;Schaub, JA;McCown, PJ;Pyle, L;Choi, YJ;Yokoo, T;Baumbach, J;Sáez, PJ;Costa, I;Turner, JE;Hodgin, JB;Saez-Rodriguez, J;Huber, TB;Bjornstad, P;Kretzler, M;Lenoir, O;Nikolic-Paterson, DJ;Pelkmans, L;Bonn, S;Puelles, VG; Pathology-oriented multiplexing enables integrative disease mapping Nature 2025-07-18 [PMID: 40681898] (IF/IHC, Human)
• Woloshuk A, Khochare S, Almulhim A et al. In situ classification of cell types in human kidney tissue using 3D nuclear staining Cytometry A 2020-11-30 [PMID: 33252180]
• Garreta E, Prado P, Tarantino C et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells Nature Materials 2019-04-01 [PMID: 30778227]
• Garreta E, Prado P, Tarantino C et al. Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells Nature Materials 2019-04-01 [PMID: 30778227]
• Nils O. Lindström, Tracy Tran, Jinjin Guo, Elisabeth Rutledge, Riana K. Parvez, Matthew E. Thornton et al. Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning Journal of the American Society of Nephrology 2018-03-01 [PMID: 29449451]
• Radmila Micanovic, Shehnaz Khan, Danielle Janosevic, Maya E. Lee, Takashi Hato, Edward F. Srour et al. Tamm-Horsfall Protein Regulates Mononuclear Phagocytes in the Kidney Journal of the American Society of Nephrology 2018-03-01 [PMID: 29180395]

Bioinformatics

• Gene Symbol: UMOD
• Uniprot:
  • P07911()