Urocortin Antibody (2D7)

Western Blot: Urocortin Antibody (2D7) [NBP2-62804] - Protein samples were separated by 16% SDS-PAGE for an hour at 200 V and electroblotted onto Immun-Blot PVDF membrane at 100 mA for 1 h. Western blotting was performed using this antibody (1:1000 dilution) as primary antibody and goat anti mouse IgG conjugated to horse radish peroxidase (1:2000). The band sizes are 35 and 80 kDa.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Urocortin Antibody (2D7) Summary

• Description: Liew, O et al, His6-SUMO-eXact tag for producing human prepro-Urocortin 2 in Escherichia coli for raising monoclonal antibodies. J Immunol Method 2014, 403:37
• Immunogen: IMAC-purified SUMO-eXact-preproUCN2
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UCN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS containing 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Protein G purified

Alternate Names for Urocortin Antibody (2D7)

• MGC129974
• MGC129975
• UCN
• UI
• UROC
• Urocortin
• urocortin, preproprotein
• UROCUI

Background

Urocortin (UCN, UCN1) is a member of the widely expressed corticotropin releasing factor (CRF) peptide family that includes UCN1-3 and CRF. UCN stimulates cAMP and MAPK pathways via either of two G-protein coupled receptors, CRFR1 and CRFR2, while other family members prefer CRFR1 (CRF) or CRFR2 (UCN2, UCN3). In the brain, UCN has been implicated in counteracting stress-provoked anxiety and suppressing appetite. It also modulates metabolic activity in peripheral tissues and protects the heart from ischemia/reprofusion injury. The UCN cDNA encodes 124 aa, including a 25 aa signal sequence, a 57 aa propeptide, and a 42 aa mature active 5 kDa UCN peptide. The proform of human UCN (aa 26-124, 11 kDa predicted) shares approximately 80% aa sequence identity with mouse and rat UCN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UCN