HAF007). A specific band was detected for Urocortin at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Urocortin Monoclonal Antibody (Catalog # MAB7447) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Urocortin at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7447-SP | |||
CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ![Western Blot: Urocortin Antibody (2D7) [NBP2-62804] - Protein samples were separated by 16% SDS-PAGE for an hour at 200 V and electroblotted onto Immun-Blot PVDF membrane at 100 mA for 1 h. Western blotting was performed using this antibody (1:1000 dilution) as primary antibody and goat anti mouse IgG conjugated to horse radish peroxidase (1:2000). The band sizes are 35 and 80 kDa.](http://images.novusbio.com/fullsize/Urocortin-Antibody-2D7-Western-Blot-NBP2-62804-img0001.jpg "Western Blot: Urocortin Antibody (2D7) [NBP2-62804] - Protein samples were separated by 16% SDS-PAGE for an hour at 200 V and electroblotted onto Immun-Blot PVDF membrane at 100 mA for 1 h. Western blotting was performed using this antibody (1:1000 dilution) as primary antibody and goat anti mouse IgG conjugated to horse radish peroxidase (1:2000). The band sizes are 35 and 80 kDa.")
