UQCRC2 Antibody

Western Blot: UQCRC2 Antibody [NBP2-20816] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with UQCRC2 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: UQCRC2 Antibody [NBP2-20816] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP2-20816] - Paraffin-embedded breast cancer. UQCRC2 antibody dilution: 1:250.

Western Blot: UQCRC2 Antibody [NBP2-20816] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:10000.

Western Blot: UQCRC2 Antibody [NBP2-20816] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunoprecipitation: UQCRC2 Antibody [NBP2-20816] - IP samples: HepG2 whole cell extract A. 35 ug HepG2 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of UQCRC2 protein by 4 ug UQCRC2 antibody 10 % SDS-PAGE. The immunoprecipitated UQCRC2 protein was detected by UQCRC2 antibody diluted at 1:5000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

• Reactivity: Hu, Mu, Rt, Pm, RM
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

UQCRC2 Antibody Summary

• Description: Novus Biologicals Rabbit UQCRC2 Antibody (NBP2-20816) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UQCRC2. The exact sequence is proprietary.
• Localization: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side
• Predicted Species: Primate (99%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UQCRC2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: Western Blot (WB) Assay-dependent dilution
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (99%), Mouse (82%), Rat (81%), Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for UQCRC2 Antibody

• Complex III subunit 2
• Core protein II
• cytochrome b-c1 complex subunit 2, mitochondrial
• QCR2
• ubiquinol-cytochrome c reductase core protein II
• Ubiquinol-cytochrome-c reductase complex core protein 2
• UQCR2

Background

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UQCRC2
• Uniprot:
  • P22695(Human)