uNOS Antibody

Immunohistochemistry-Paraffin: uNOS Antibody [NBP2-42208] - Human brain in multiple sclerosis lesion. Image from verified customer review.

Immunohistochemistry-Paraffin: uNOS Antibody [NBP2-42208] - Human placenta stained with Anti-uNOS antibody

Product Discontinued

uNOS Antibody Summary

• Immunogen: Synthetic peptide (Mouse) (C terminal).
• Localization: Cytoplasmic
• Specificity: This reacts with iNOS, bNOS and eNOS.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

May cross-react with Mouse, Rat, Cow and Pig. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.6) and 1.0% BSA
• Preservative: 0.1% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for uNOS Antibody

• bNOS
• Constitutive NOS
• IHPS 1
• IHPS1
• N NOS
• NC NOS
• Neuronal nitric oxide synthase
• Neuronal NOS
• Nitric oxide synthase 1 neuronal
• Nitric oxide synthase 1
• Nitric oxide synthase brain
• nNOS
• Nos 1
• NOS type I
• NOS
• NOS1

Background

NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated, acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOS2
• Entrez:
  • 4843(Human)
  • 24599(Rat)