uNOS Antibody (NOS-3F7-B11 B5) Summary
Purified bovine bNOS.
Detects brain Nitric Oxide Synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS).
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:50 - 1:500
- Immunocytochemistry/Immunofluorescence 1:20
- Immunohistochemistry 1:10 - 1:500
- Immunohistochemistry-Frozen 1:100
- Immunoprecipitation 1:10 - 1:500
WB: Detects an approx. 130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an approx. 155 kDa band in tissues expressing bNOS and an approx. 140 kDa band in tissues expressing eNOS.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Alternate Names for uNOS Antibody (NOS-3F7-B11 B5)
- Constitutive NOS
- IHPS 1
- N NOS
- NC NOS
- Neuronal nitric oxide synthase
- Neuronal NOS
- Nitric oxide synthase 1 neuronal
- Nitric oxide synthase 1
- Nitric oxide synthase brain
- Nos 1
- NOS type I
Nitric Oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells including vasorelaxation, neurotransmission and cytotoxicity. NO is produced by a Nitric Oxide Synthase (NOS) family of enzymes which oxidizes the amino acid L-arginine to form L-citrulline and NO.Two constitutive forms, brain NOS (bNOS) & endothelial cell NOS (eNOS), and one inducible form (iNOS), have been cloned showing regions for binding of calmodulin, NADPH, FAD, and FMN. iNOS has been found in a variety of tissues including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. It is induced after exposure to cytokines such as interferon-* (IFN), tumor necrosis factor, interleukin-1, interleukin-2, and lipopolysaccarides (LPS). After cytokine induction, iNOS has a delayed response followed by high levels of NO produced over a long period of time. eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa & pancreatic islet cells. eNOS is capable of synthesizing NO in vascular endothelial cells where it appears to play an important role in the control of vasotension and platelet aggregation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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