uNOS Antibody (NOS-3F7-B11 B5)

Images

 
Western Blot: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Analysis of 25 ug of mouse brain (lane 1) and C2C12 (lane 2) lysates.
Immunocytochemistry/ Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a uNOS ...read more
Immunocytochemistry/ Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% ...read more
Immunocytochemistry/ Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% ...read more
Immunocytochemistry/ Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP
Clone
NOS-3F7-B11 B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

uNOS Antibody (NOS-3F7-B11 B5) Summary

Immunogen
Purified bovine bNOS.
Specificity
Detects brain Nitric Oxide Synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS).
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
NOS2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:20
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an approx. 155 kDa band in tissues expressing bNOS and an approx. 140 kDa band in tissues expressing eNOS.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for uNOS Antibody (NOS-3F7-B11 B5)

  • bNOS
  • Constitutive NOS
  • IHPS 1
  • IHPS1
  • N NOS
  • NC NOS
  • Neuronal nitric oxide synthase
  • Neuronal NOS
  • Nitric oxide synthase 1 neuronal
  • Nitric oxide synthase 1
  • Nitric oxide synthase brain
  • nNOS
  • Nos 1
  • NOS type I
  • NOS
  • NOS1

Background

Nitric Oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells including vasorelaxation, neurotransmission and cytotoxicity. NO is produced by a Nitric Oxide Synthase (NOS) family of enzymes which oxidizes the amino acid L-arginine to form L-citrulline and NO.Two constitutive forms, brain NOS (bNOS) & endothelial cell NOS (eNOS), and one inducible form (iNOS), have been cloned showing regions for binding of calmodulin, NADPH, FAD, and FMN. iNOS has been found in a variety of tissues including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. It is induced after exposure to cytokines such as interferon-* (IFN), tumor necrosis factor, interleukin-1, interleukin-2, and lipopolysaccarides (LPS). After cytokine induction, iNOS has a delayed response followed by high levels of NO produced over a long period of time. eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa & pancreatic islet cells. eNOS is capable of synthesizing NO in vascular endothelial cells where it appears to play an important role in the control of vasotension and platelet aggregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Array NB120-2801

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Bioinformatics

Gene Symbol NOS2
Entrez