UNC13D/Munc 13-4 Antibody

Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - RBL-2H3 from Wt or shRNA Munc13-4 lentiviral stable expression whole cell lysate. 50 ug total protein. WB image submitted by a verified customer review.

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Mouse Lung with antibody at 0.5 ug/mL (wt in A and KO in B).

Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of Human T-lymphocyte lysate with antibody at 2 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Human Thymus. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA were left untreated (Untr) or were treated with TGFbeta-1 for 24 h, and SDS-PAGE Western blotting for indicated proteins was conducted. Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY licence.

Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA, KD
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UNC13D/Munc 13-4 Antibody Summary

• Immunogen: Peptide with sequence C-ETQKHKKDLHPLFD corresponding to internal region according to NP_954712.1.
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UNC13D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 3 ug/mL
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Knockdown Validated
• Application Notes: WB: Approx. 105 kDa band observed in human T-lymphocyte and HeLa lysates (calculated MW of 123 kDa band according to NP_954712.1). IHC-P: Human thymus shows cytoplasm staining in select areas. Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out, provided by Micheal Tuvim, University of Texas MD Anderson Cancer Center, Houston, USA. Use in ICC/IF reported in scientific literature (PMID 28100639).
• Control:

  UNC13D/Munc 13-4 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UNC13D/Munc 13-4 Antibody

• FHL3
• HLH3
• HPLH3
• Munc13-4
• Munc13-4protein unc-13 homolog D
• unc-13 homolog D (C. elegans)
• UNC13D

Background

This gene encodes a protein that is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis type 3, a genetically heterogeneous, rare autosomal recessive disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UNC13D/Munc 13-4 Antibody (NB100-41385)(5)

Publications using NB100-41385

• Messenger SW, Woo SS, Sun Z, Martin TFJ. A Ca2+-stimulated exosome release pathway in cancer cells is regulated by Munc13-4 J. Cell Biol. Jun 21 2018 [PMID: 29930202] (WB, Human)
• Woo SS, James DJ, Martin TF. Munc13-4 functions as a Ca2+ sensor for homotypic secretory granule fusion to generate endosomal exocytic vacuoles Mol. Biol. Cell Jan 18 2017 [PMID: 28100639] (ICC/IF, Human)
• Zur Stadt U, Beutel K, Kolberg S et al. Mutation spectrum in children with primary hemophagocytic lymphohistiocytosis: molecular and functional analyses of PRF1, UNC13D, STX11, and RAB27A. Hum Mutat 2006 Jan [PMID: 16278825]
• Man KNM. Role of Munc13 Isoforms in Regulating Large Dense Core Vesicle Exocytosis in Chromaffin Cells. Thesis. (WB, Mouse)
• Man KN, Imig C, Walter AM et al. Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis. Elife 2015 Nov 17 [PMID: 26575293] (WB)

Review for UNC13D/Munc 13-4 Antibody (NB100-41385) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Scott Messenger WB Rat 04/14/2017

Summary

• Application: Western Blot
• Sample Tested: RBL-2H3 rat basophilic leukemia cell line
• Species: Rat

Comments

• Comments: RBL-2H3 from Wt or shRNA Munc13-4 lentiviral stable expression whole cell lysate. 50ug protein

FAQs for UNC13D/Munc 13-4 Antibody (NB100-41385). (Showing 1 - 1 of 1 FAQs).

1. I'm looking Munc13-4 N terminal antibody with related epitope sequence.
   • Unfortunately we do not epitope map our antibodies, but we do have a whole list of antibodies against your target depending on species reactivity and application you want to use them for. Please see this link to our MUNC 13-4 antibodies. All of the immunogen information that is not proprietary, or known should be presented on the datasheet. Often times a range will be provided where the peptide falls within, or if it was raised against a larger recombinant protein as is the case for the one you inquired about we do not know exactly where the binding occurs.

Bioinformatics

• Gene Symbol: UNC13D
• Entrez:
  • 201294(Human)
  • 70450(Mouse)
  • 192177(Rat)
• Uniprot:
  • NP_954712.1(Human)