UNC13D/Munc 13-4 Antibody

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-1434] - Staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UNC13D/Munc 13-4 Antibody Summary

• Immunogen: Peptide with sequence C-KQASQHALRPAP corresponding to C-Terminus according to NP_954712.1.
• Localization: Cell Membrane and Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UNC13D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1:100-1:2000
• Application Notes: This antibody has been successfully used in Western blot in Human.
• Control:

  UNC13D/Munc 13-4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UNC13D/Munc 13-4 Antibody

• FHL3
• HLH3
• HPLH3
• Munc13-4
• Munc13-4protein unc-13 homolog D
• unc-13 homolog D (C. elegans)
• UNC13D

Background

Secretion of the contents of cytolytic granules at the immunologic synapse is a highly regulated process essential for lymphocyte cytotoxicity. This process requires the rapid transfer of perforin containing lytic granules to the target cell interface, followed by their docking and fusion with the plasma membrane. Munc 13-4 localises with cytotoxic granules at the immunologic synapse. Munc 13-4 is essential for the priming step of cytolytic granule secretion preceding vesicle membrane fusion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UNC13D/Munc 13-4 Antibody (NB100-1434)(1)

Publication using NB100-1434

• Feldmann J, Callebaut I, Raposo G et al. Munc13-4 is essential for cytolytic granules fusion and is mutated in a form of familial hemophagocytic lymphohistiocytosis (FHL3). Cell 2003-11-14 [PMID: 14622600]

FAQs for UNC13D/Munc 13-4 Antibody (NB100-1434). (Showing 1 - 1 of 1 FAQ).

1. I'm looking Munc13-4 N terminal antibody with related epitope sequence.
   • Unfortunately we do not epitope map our antibodies, but we do have a whole list of antibodies against your target depending on species reactivity and application you want to use them for. Please see this link to our MUNC 13-4 antibodies. All of the immunogen information that is not proprietary, or known should be presented on the datasheet. Often times a range will be provided where the peptide falls within, or if it was raised against a larger recombinant protein as is the case for the one you inquired about we do not know exactly where the binding occurs.

Bioinformatics

• Gene Symbol: UNC13D
• Entrez:
  • 201294(Human)
• Uniprot:
  • NP_954712.(Human)