ULBP-3 Antibody (166514)

• Reactivity: Hu
• Applications: WB
• Clone: 166514
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ULBP-3 Antibody (166514) Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human ULBP-3
  Accession # Q9BZM4
• Specificity: Detects human ULBP-3 in direct ELISAs and Western blots. In these formats, this antibody does not cross‑react with recombinant human (rh) ULBP-1 or rhULBP-2.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ULBP3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-3 Antibody (166514)

• NKG2D ligand 3
• RAET1N
• UL16 binding protein 3
• ULBP3
• ULBP-3

Background

ULBP-3 is a GPI-linked cell-surface protein that functions as a ligand for human NKG2D. ULBP-1, -2, and -3 are expressed on some tumor cells and have been implicated in immune surveillance of tumors. Unlike ULBP-1 and -2, ULBP-3 does not interact with the CMV glycoprotein UL16.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ULBP-3 Antibody (MAB15171)(7)

Publications using MAB15171

• Abigael Eva Chaouat, Barbara Seliger, Ofer Mandelboim, Dominik Schmiedel The HHV-6A Proteins U20 and U21 Target NKG2D Ligands to Escape Immune Recognition Frontiers in Immunology 10/15/2021 [PMID: 34721381]
• E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 2021-05-03;17(5):e1008807. 2021-05-03 [PMID: 33939764] (Flow Cytometry, Human)
• E Seidel, L Dassa, S Kahlon, B Tirosh, A Halenius, T Seidel Mal, O Mandelboim A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain Nature Communications, 2021-04-06;12(1):2061. 2021-04-06 [PMID: 33824318] (Flow Cytometry, Human)
• M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D Viruses, 2020-07-20;12(7):. 2020-07-20 [PMID: 32698530] (Flow Cytometry, Human)
• Costa D, Vene R, Benelli R et al. Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts Front Immunol 5/29/2018 [PMID: 29910806]
• Maria Raffaella Zocchi, Silvia Catellani, Paolo Canevali, Sara Tavella, Anna Garuti, Barbara Villaggio et al. High ERp5/ADAM10 expression in lymph node microenvironment and impaired NKG2D ligands recognition in Hodgkin lymphomas Blood 2/9/2012 [PMID: 22167753]
• Poggi A, Catellani S, Garuti A, Pierri I, Gobbi M, Zocchi MR Effective in vivo induction of NKG2D ligands in acute myeloid leukaemias by all-trans-retinoic acid or sodium valproate. Leukemia, 2009-01-08;23(4):641-8. 2009-01-08 [PMID: 19151770] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: ULBP3
• Uniprot:
  • Q9BZM4()