ULBP-2/5/6 Antibody (165903) [Unconjugated]

Images

 
HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 Monoclonal Antibody (Catalog # MAB1298, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready
Clone
165903
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

ULBP-2/5/6 Antibody (165903) [Unconjugated] Summary

Immunogen
BaF3 mouse pro-B cell line transfected with human ULBP-2
Accession # Q9BZM5
Specificity
Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction 1 ug/mL
  • CyTOF-ready
Publications
Read Publications using
MAB1298 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-2/5/6 Antibody (165903) [Unconjugated]

  • ALCAN-alpha
  • N2DL2
  • NKG2DL2
  • RAET1H
  • RAET1L
  • UL16 binding protein 2
  • ULBP2
  • ULBP-2/5/6

Background

ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.
  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114.
  8. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ULBP-2/5/6 Antibody (MAB1298)(41)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: ELISA Development, ELISA Development (Detection), Flow Cytometry, Functional Assay, ICC, Immunoprecipitation, Neutralization.


Filter By Application
ELISA Development
(3)
ELISA Development (Detection)
(2)
Flow Cytometry
(31)
Functional Assay
(1)
ICC
(1)
Immunoprecipitation
(1)
Neutralization
(2)
All Applications
Filter By Species
Human
(40)
All Species
Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using MAB1298 Applications Species
M Anft, P Netter, D Urlaub, I Prager, S Schaffner, C Watzl NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production Cell. Mol. Immunol., 2019;0(0):. 2019 [PMID: 31471588] (Flow Cytometry, Human) Flow Cytometry Human
M Cucè, ME Gallo Cant, MA Siciliano, C Riillo, D Caracciolo, F Scionti, N Staropoli, V Zuccalà, L Maltese, A Di Vito, K Grillone, V Barbieri, M Arbitrio, MT Di Martino, M Rossi, N Amodio, P Tagliaferr, P Tassone, C Botta Trabectedin triggers direct and NK-mediated cytotoxicity in multiple myeloma J Hematol Oncol, 2019;12(1):32. 2019 [PMID: 30898137] (Flow Cytometry, Human) Flow Cytometry Human
YS Lee, W Heo, J Nam, YH Jeung, J Bae The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells J. Radiat. Res., 2018;0(0):. 2018 [PMID: 29518205] (Flow Cytometry, Human) Flow Cytometry Human
S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors Oncoimmunology, 2018;7(2):e1392426. 2018 [PMID: 29308322] (Flow Cytometry, Human) Flow Cytometry Human
MT McCarthy, G Moncayo, TK Hiron, NA Jakobsen, A Valli, T Soga, J Adam, CA O'Callagha Purine nucleotide metabolism regulates expression of the human immune ligand MICA J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29279329] (Flow Cytometry, Human) Flow Cytometry Human
Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20 Sci Rep, 2017;7(1):8661. 2017 [PMID: 28819195] (Flow Cytometry, Human) Flow Cytometry Human
C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells Cell Death Dis, 2017;8(8):e2973. 2017 [PMID: 28771222] (Flow Cytometry, Human) Flow Cytometry Human
NA Zumwalde, A Sharma, X Xu, S Ma, CL Schneider, JC Romero-Mas, AW Hudson, A Gendron-Fi, SC Kenney, JE Gumperz Adoptively transferred V?9V?2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model JCI Insight, 2017;2(13):. 2017 [PMID: 28679955] (Flow Cytometry, Human) Flow Cytometry Human
X Cai, A Caballero-, MM Gewe, IC Jenkins, CW Drescher, RK Strong, T Spies, V Groh Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells Neoplasia, 2017;19(6):471-482. 2017 [PMID: 28499126] (Functional Assay, Human) Functional Assay Human
S Weil, S Memmer, A Lechner, V Huppert, A Giannattas, T Becker, A Müller-Run, K Lampe, D Beutner, A Quaas, R Schubert, E Herrmann, A Steinle, U Koehl, L Walter, MS von Bergwe, J Koch Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma Front Immunol, 2017;8(0):387. 2017 [PMID: 28443091] (ELISA Development (Detection)) ELISA Development (Detection)
Show All 41 Publications.

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