HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 Monoclonal Antibody (Catalog # MAB1298, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by ...read more
BaF3 mouse pro-B cell line transfected with human ULBP-2 Accession # Q9BZM5
Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ULBP-2/5/6 Antibody (165903) [Unconjugated]
UL16 binding protein 2
multiple signaling pathways in primary NK cells, resulting in the production of
cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium
mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal
transduction pathway. The name ULBP derives from the original identification of
three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus
glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes
for ULBPs reside in a cluster of ten related genes, six of which encode
potentially functional glycoproteins. ULBP-2 has also been described under the
names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5
also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are
distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2
Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin.
Some family members, including ULBP-2, are anchored to the membrane via a
GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D
results in the activation of cytolytic activity and/or cytokine production by
these effector cells. The ULBPs are expressed on some tumor cells and have been
implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and
95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.
Cosman, D. et al. (2001) Immunity 14:123.
Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
Sutherland, C. et al. (2002) J. Immunol. 168:671.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
Pende, D. et al. (2002) Cancer Res. 62:6178.
Radosavljevic, M. et al. (2002) Genomics 79:114.
NKG2D and its Ligands (2002) www.RnDSystems.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.