ULBP-2/5/6 Antibody (165903) [Allophycocyanin]

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 APC-conjugated Monoclonal Antibody (Catalog # FAB1298A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Histograms showing sample flow cytometry profiles for the ligands studied. Bars in each plot indicate the population expressing each aNKR, Gates were set using Unstained, single stained controls, fluorescence minus one, and secondary antibody alone controls. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1999-4915/9/10/295), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 165903
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

ULBP-2/5/6 Antibody (165903) [Allophycocyanin] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human ULBP-2
  Accession # Q9BZM5
• Specificity: Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-2/5/6 Antibody (165903) [Allophycocyanin]

• ALCAN-alpha
• N2DL2
• NKG2DL2
• RAET1H
• RAET1L
• UL16 binding protein 2
• ULBP2
• ULBP-2/5/6

Background

ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.

1. Cosman, D. et al. (2001) Immunity 14:123.
2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
4. Steinle, A. et al. (2001) Immunogenetics 53:279.
5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. Radosavljevic, M. et al. (2002) Genomics 79:114.
8. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ULBP-2/5/6 Antibody (FAB1298A)(23)

Publications using FAB1298A

• Li, YR;Fang, Y;Niu, S;Zhu, Y;Chen, Y;Lyu, Z;Zhu, E;Tian, Y;Huang, J;Rezek, V;Kitchen, S;Hsiai, T;Zhou, JJ;Wang, P;Chai-Ho, W;Park, S;Seet, CS;Oliai, C;Yang, L; Allogeneic CD33-directed CAR-NKT cells for the treatment of bone marrow-resident myeloid malignancies Nature communications 2025-02-01 [PMID: 39893165] (Flow Cytometry, Human)
• Zhao, NQ;Pi, R;Nguyen, DN;Ranganath, T;Seiler, C;Holmes, S;Marson, A;Blish, CA; NKp30 and NKG2D contribute to natural killer recognition of HIV-infected cells bioRxiv : the preprint server for biology 2024-06-27 [PMID: 38979175] (Flow Cytometry, Human)
• Aublin-Gex, A;Jacolin, F;Diaz, O;Jacquemin, C;Marçais, A;Walzer, T;Lotteau, V;Vidalain, PO;Perrin-Cocon, L; Tethering of hexokinase 2 to mitochondria promotes resistance of liver cancer cells to natural killer cell cytotoxicity European journal of immunology 2024-06-04 [PMID: 38837415] (Flow Cytometry, Human)
• Yan-Ruide Li, Yang Zhou, Yu Jeong Kim, Yanni Zhu, Feiyang Ma, Jiaji Yu et al. Development of allogeneic HSC-engineered iNKT cells for off-the-shelf cancer immunotherapy Cell Reports Medicine 2021-11-16 [PMID: 34841295] (Flow Cytometry, Human)
• R Fu, F Yu, W Wu, J Liu, J Li, F Guo, L Xu, F Wang, X Cui Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding International immunopharmacology, 2021-10-19;101(0):108195. 2021-10-19 [PMID: 34678691] (Flow Cytometry, Human)
• X Zhong, H Liao, S Hu, K Luo, H Zhu The diterpenoid adenanthin upregulates the expression of natural killer group 2D receptor ligands in hepatocellular carcinoma cells Molecular and cellular probes, 2021-07-12;59(0):101759. 2021-07-12 [PMID: 34265372] (Flow Cytometry, Human)
• RI Høgh, A Droujinine, SH Møller, SD Jepsen, M Mellergaar, L Andresen, S Skov Fumarate Upregulates Surface Expression of ULBP2/ULBP5 by Scavenging Glutathione Antioxidant Capacity J. Immunol., 2020-03-06;0(0):. 3/6/2020 [PMID: 32144161]
• D Suzuki, C Flahou, N Yoshikawa, I Stirblyte, Y Hayashi, A Sawaguchi, M Akasaka, S Nakamura, N Higashi, H Xu, T Matsumoto, K Fujio, MG Manz, A Hotta, H Takizawa, K Eto, N Sugimoto iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity Stem Cell Reports, 2019-12-26;0(0):. 2019-12-26 [PMID: 31883921] (Flow Cytometry, Human)
• N Mallmann-G, Y Sax, R Kimmig, S Lang, S Brandau EGFR-Specific Tyrosine Kinase Inhibitor Modifies NK Cell-Mediated Antitumoral Activity against Ovarian Cancer Cells Int J Mol Sci, 2019-09-22;20(19):. 2019-09-22 [PMID: 31546690] (Flow Cytometry, Human)
• M Chollat-Na, T Ben Safta-, D Haferssas, G Meurice, S Chouaib, J Thiery The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy Cell Death Dis, 2019-09-20;10(10):695. 2019-09-20 [PMID: 31541080] (Flow Cytometry, Human)
• Jaydeep Bhat, Samuel Dubin, Alexandra Dananberg, Elgar Susanne Quabius, Juergen Fritsch, C. Marie Dowds et al. Histone Deacetylase Inhibitor Modulates NKG2D Receptor Expression and Memory Phenotype of Human Gamma/Delta T Cells Upon Interaction With Tumor Cells Frontiers in Immunology 2019-03-27 [PMID: 30972064]
• A Tremblay-M, S Coenraads, Z Kiani, FP Dupuy, NF Bernard Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function BMC Immunol., 2019-01-29;20(1):8. 2019-01-29 [PMID: 30696399] (Flow Cytometry, Human)
• Eytan Breman, Benjamin Demoulin, Sophie Agaugué, Sebastien Mauën, Alexandre Michaux, Lorraine Springuel et al. Overcoming Target Driven Fratricide for T Cell Therapy Frontiers in Immunology 2018-12-12 [PMID: 30619300]
• AT Björklund, M Carlsten, E Sohlberg, LL Liu, T Clancy, M Karimi, S Cooley, JS Miller, M Klimkowska, M Schaffer, E Watz, KI Wikstrom, P Blomberg, BE Wahlin, M Palma, L Hansson, P Ljungman, E Hellström-, HG Ljunggren, KJ Malmberg Complete Remission with Reduction of High-risk Clones following Haploidentical NK Cell Therapy against MDS and AML Clin. Cancer Res., 2018-02-14;0(0):. 2018-02-14 [PMID: 29444931] (Flow Cytometry, Human)
• Ryoji Kamei, Kiyoshi Yoshimura, Shigefumi Yoshino, Moeko Inoue, Tetsuhiko Asao, Masanori Fuse et al. Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy Oncology Letters 2017-11-06 [PMID: 29391893] (Flow Cytometry, Human)
• A Tremblay-M, J Bruneau, B Lebouché, I Lisovsky, R Song, NF Bernard Expression Profiles of Ligands for Activating Natural Killer Cell Receptors on HIV Infected and Uninfected CD4? T Cells Viruses, 2017-10-12;9(10):. 2017-10-12 [PMID: 29023371] (Flow Cytometry, Human)
• Wei-Chen Huang T Cells Infiltrating Diseased Liver Express Ligands for the NKG2D Stress Surveillance System J. Immunol, 2016-12-28;0(0):. 2016-12-28 [PMID: 28031333] (Flow Cytometry, Human)
• Leung W, Vong Q, Lin W, Bouck D, Wendt S, Sullivan E, Li Y, Bari R, Chen T, Leung W PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60. J Immunol, 2015-02-16;194(6):2930-41. 2015-02-16 [PMID: 25687758] (Flow Cytometry, Human)
• Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway. J Immunol, 2014-07-14;193(4):1654-65. 2014-07-14 [PMID: 25024379] (Flow Cytometry, Human)
• Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation. J Exp Med, 2013-11-04;210(12):2675-92. 2013-11-04 [PMID: 24190430] (Flow Cytometry, Human)
• Madrid , Alexis S, Ganem , Don Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44. J Virol, 2012-06-06;86(16):8693-704. 2012-06-06 [PMID: 22674989] (Flow Cytometry, Human)
• Anindya Dasgupta, Jordan E. Shields, H. Trent Spencer Treatment of a Solid Tumor Using Engineered Drug-Resistant Immunocompetent Cells and Cytotoxic Chemotherapy Human Gene Therapy 2012-07-01 [PMID: 22397715]
• Jensen H, Andresen L, Nielsen J, Christensen JP, Skov S Vesicular Stomatitis Virus Infection Promotes Immune Evasion by Preventing NKG2D-Ligand Surface Expression. PLoS ONE, 2011-08-09;6(8):e23023. 2011-08-09 [PMID: 21857986] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • Q9BZM5()