ULBP-2/5/6 Antibody (165903) [Phycoerythrin]

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 PE-conjugated Monoclonal Antibody (Catalog # FAB1298P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 165903
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

ULBP-2/5/6 Antibody (165903) [Phycoerythrin] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human ULBP-2
  Accession # Q9BZM5
• Specificity: Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-2/5/6 Antibody (165903) [Phycoerythrin]

• ALCAN-alpha
• N2DL2
• NKG2DL2
• RAET1H
• RAET1L
• UL16 binding protein 2
• ULBP2
• ULBP-2/5/6

Background

ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.

1. Cosman, D. et al. (2001) Immunity 14:123.
2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
4. Steinle, A. et al. (2001) Immunogenetics 53:279.
5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. Radosavljevic, M. et al. (2002) Genomics 79:114.
8. NKG2D and its Ligands (2002) www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ULBP-2/5/6 Antibody (FAB1298P)(35)

Publications using FAB1298P

• He, X;Wang, L;Zeng, W;Wang, Y;Chen, N;Yang, P;Ti, A;Zhang, Q;Shao, Y;Wang, M;Huang, Z;Zhang, X;Xu, M;Liang, L;Wang, X;Ding, X;Zhu, T;Zhang, P;Pan, Z;Yang, F;Zhou, Y;Mo, G;Hu, J;Yue, Y;Hu, J;Deng, Y;Ho, T;Church, GM;Hu, Y;Huang, H;Yang, L; Regulatable C-X-C chemokine receptor type 4 in iPSC-derived NK cells improves bone marrow chemotaxis and targeting resident tumor Trends in biotechnology 2025-04-30 [PMID: 40312161] (Human)
• Lee, S;Chae, SJ;Jang, IH;Oh, SC;Kim, SM;Lee, SY;Kim, JH;Ko, J;Kim, HJ;Song, IC;Kim, JK;Kim, TD; B7H6 is the predominant activating ligand driving natural killer cell-mediated killing in patients with liquid tumours: evidence from clinical, in silico, in vitro, and in vivo studies EBioMedicine 2024-11-22 [PMID: 39579618] (FACS, Human)
• Liu, J;Xing, L;Li, J;Wen, K;Liu, N;Liu, Y;Wu, G;Wang, S;Ogiya, D;Song, TY;Kurata, K;Penailillo, J;Morelli, E;Wang, T;Hong, X;Gulla, A;Tai, YT;Munshi, N;Richardson, P;Carrasco, R;Hideshima, T;Anderson, KC; Epigenetic regulation of CD38/CD48 by KDM6A mediates NK cell response in multiple myeloma Nature communications 2024-02-14 [PMID: 38355622] (Flow Cytometry, Human)
• Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS; Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer Oncology letters 2023-06-01 [PMID: 37153058] (Flow Cytometry, Human)
• NL de Vries, J van de Haa, V Veninga, M Chalabi, ME Ijsselstei, M van der Pl, J van den Bu, D Ruano, JG van den Be, JB Haanen, LJ Zeverijn, BS Geurts, GF de Wit, TW Battaglia, H Gelderblom, HMW Verheul, TN Schumacher, LFA Wessels, F Koning, NFCC de Miranda, EE Voest gammadelta T cells are effectors of immunotherapy in cancers with HLA class I defects Nature, 2023-01-11;613(7945):743-750. 2023-01-11 [PMID: 36631610] (Flow Cytometry, Human)
• Dario Gerace, Quan Zhou, Jennifer Hyoje-Ryu Kenty, Adrian Veres, Elad Sintov, Xi Wang et al. Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance Cell Reports Medicine 2023-01-17 [PMID: 36599351]
• R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement Cell Reports, 2022-09-27;40(13):111423. 2022-09-27 [PMID: 36170817] (ICC, Human)
• Y Murayama, Y Kasahara, N Kubo, C Shin, M Imamura, N Oike, T Ariizumi, A Saitoh, M Baba, T Miyazaki, Y Suzuki, Y Ling, S Okuda, K Mihara, A Ogose, H Kawashima, C Imai NKp44-based chimeric antigen receptor effectively redirects primary T cells against synovial sarcoma Translational Oncology, 2022-08-20;25(0):101521. 2022-08-20 [PMID: 35998437] (Flow Cytometry, Human)
• D Bernareggi, Q Xie, BC Prager, J Yun, LS Cruz, TV Pham, W Kim, X Lee, M Coffey, C Zalfa, P Azmoon, H Zhu, P Tamayo, JN Rich, DS Kaufman CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity Nature Communications, 2022-04-07;13(1):1899. 2022-04-07 [PMID: 35393416] (Flow Cytometry, Human)
• T Seki, Y Shimizu, K Ishii, Y Takahama, K Kato, T Yano NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway Biomolecules, 2021-11-16;11(11):. 2021-11-16 [PMID: 34827699] (Flow Cytometry, Human)
• Van der Meer JMR, de Jonge PKJD, van der Waart AB et al. CD34(+) progenitor-derived NK cell and gemcitabine combination therapy increases killing of ovarian cancer cells in NOD/SCID/IL2Rg(null) mice OncoImmunology 2021-10-07 [PMID: 34616589]
• Y Zhu, J Xie, J Shi Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis Bmc Biology, 2021-07-30;19(1):140. 2021-07-30 [PMID: 34325694] (Flow Cytometry, Human)
• Ana L. Portillo, Richard Hogg, Sophie M. Poznanski, Eduardo A. Rojas, Niamh J. Cashell, Joanne A. Hammill et al. Expanded human NK cells armed with CAR uncouple potent anti-tumor activity from off-tumor toxicity against solid tumors iScience 2021-06-25 [PMID: 34159300]
• Thibault Carré, Jerome Thiery, Bassam Janji, Stéphane Terry, Gwendoline Gros, Guillaume Meurice et al. Selection of tumor‑resistant variants following sustained natural killer cell‑mediated immune stress Oncology Reports 2020-11-27 [PMID: 33416152]
• Benjamin H. Goldenson, Huang Zhu, YunZu Michele Wang, Naveen Heragu, Davide Bernareggi, Alessa Ruiz-Cisneros et al. Umbilical Cord Blood and iPSC-Derived Natural Killer Cells Demonstrate Key Differences in Cytotoxic Activity and KIR Profiles Frontiers in Immunology 2020-10-15 [PMID: 33178188]
• V Ponath, M Frech, M Bittermann, R Al Khayer, A Neubauer, C Brendel, E Pogge von The Oncoprotein SKI Acts as A Suppressor of NK Cell-Mediated Immunosurveillance in PDAC Cancers (Basel), 2020-10-03;12(10):. 2020-10-03 [PMID: 33023028] (Flow Cytometry, Human)
• Sofie H. Møller, Maiken Mellergaard, Mikkel Madsen, Amaia V. Bermejo, Stine D. Jepsen, Marie H. Hansen et al. Cytoplasmic Citrate Flux Modulates the Immune Stimulatory NKG2D Ligand MICA in Cancer Cells Frontiers in Immunology 2020-08-11 [PMID: 32849657]
• DR Makanga, F Da Rin de, G David, C Willem, L Dubreuil, N Legrand, T Guillaume, P Peterlin, A Lebourgeoi, MC Béné, A Garnier, P Chevallier, K Gendzekhad, A Cesbron, K Gagne, B Clemenceau, C Retière Genetic and Molecular Basis of Heterogeneous NK Cell Responses against Acute Leukemia Cancers (Basel), 2020-07-16;12(7):. 2020-07-16 [PMID: 32708751] (Flow Cytometry, Human)
• Frank Fasbender, Martin Obholzer, Sarah Metzler, Regina Stöber, Jan G. Hengstler, Carsten Watzl Enhanced activation of human NK cells by drug-exposed hepatocytes Archives of Toxicology 2020-02-01 [PMID: 32060585]
• VA Jennings, GB Scott, AMS Rose, KJ Scott, G Migneco, B Keller, K Reilly, O Donnelly, H Peach, D Dewar, KJ Harrington, H Pandha, A Samson, RG Vile, AA Melcher, F Errington- Potentiating Oncolytic Virus-Induced Immune-Mediated Tumor Cell Killing Using Histone Deacetylase Inhibition Mol. Ther., 2019-04-14;0(0):. 2019-04-14 [PMID: 31053413] (Flow Cytometry, Human)
• Masami Iwasa, Takeshi Harada, Asuka Oda, Ariunzaya Bat-Erdene, Jumpei Teramachi, Hirofumi Tenshin et al. PD-L1 upregulation in myeloma cells by panobinostat in combination with interferon-gamma Oncotarget 2019-03-08 [PMID: 30956773] (Flow Cytometry, Human)
• Y Han, W Xie, DG Song, DJ Powell Control of triple-negative breast cancer using ex vivo self-enriched, costimulated NKG2D CAR T cells J Hematol Oncol, 2018-07-06;11(1):92. 2018-07-06 [PMID: 29980239] (CAR-T (Flow Cytometry), Human)
• JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells Oncotarget, 2017-01-01;0(0):. 2017-01-01 [PMID: 28615524] (Flow Cytometry, Human)
• Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand PLoS Pathog, 2016-12-01;12(12):e1006015. 2016-12-01 [PMID: 27907183] (Flow Cytometry, Human)
• Xiansheng Lin, Mei Huang, Fang Xie, Hangcheng Zhou, Ji Yang, Qiang Huang Gemcitabine inhibits immune escape of pancreatic cancer by down regulating the soluble ULBP2 protein Oncotarget 2016-10-25 [PMID: 27602753]
• Decitabine enhances anti-CD33 monoclonal antibody BI 836858-mediated natural killer ADCC against AML blasts Blood, 2016-03-24;127(23):2879-89. 2016-03-24 [PMID: 27013443] (Flow Cytometry, Human)
• Lee S, Kang W, Yoon Y, Jin J, Song H, Her J, Kang S, Hwang Y, Kang K, Joo K, Nam D Natural killer (NK) cells inhibit systemic metastasis of glioblastoma cells and have therapeutic effects against glioblastomas in the brain. BMC Cancer, 2015-12-24;15(1):1011. 2015-12-24 [PMID: 26704632] (Flow Cytometry, Human)
• Parrish C, Scott G, Migneco G, Scott K, Steele L, Ilett E, West E, Hall K, Selby P, Buchanan D, Varghese A, Cragg M, Coffey M, Hillmen P, Melcher A, Errington-Mais F Oncolytic reovirus enhances rituximab-mediated antibody-dependent cellular cytotoxicity against chronic lymphocytic leukaemia. Leukemia, 2015-03-27;29(9):1799-810. 2015-03-27 [PMID: 25814029] (Flow Cytometry, Human)
• Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway. J Immunol, 2014-07-14;193(4):1654-65. 2014-07-14 [PMID: 25024379] (Flow Cytometry, Human)
• Wang , Ruipeng, Sun , Peter D Natural killer cell-mediated shedding of ULBP2. PLoS ONE, 2014-03-10;9(3):e91133. 2014-03-10 [PMID: 24614922] (Flow Cytometry, Neutralization, Human)
• Sandra A. Jacobs, Jeroen Plessers, Jef Pinxteren, Valerie D. Roobrouck, Catherine M. Verfaillie, Stefaan W. Van Gool Mutual Interaction between Human Multipotent Adult Progenitor Cells and NK Cells Cell Transplantation 2014-09-01 [PMID: 23562064]
• Wang R, Jaw JJ, Jaw J, Stutzman NC, Stutzman N, Zou Z, Sun PD Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1. J Leukoc Biol, 2011-11-01;91(2):299-309. 2011-11-01 [PMID: 22045868] (Flow Cytometry, Human)
• Jensen H, Andresen L, Nielsen J, Christensen JP, Skov S Vesicular Stomatitis Virus Infection Promotes Immune Evasion by Preventing NKG2D-Ligand Surface Expression. PLoS ONE, 2011-08-09;6(8):e23023. 2011-08-09 [PMID: 21857986] (Flow Cytometry, Human)
• Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells. PLoS ONE, 2011-02-25;6(2):e16899. 2011-02-25 [PMID: 21364924] (Flow Cytometry, Human)
• Cho D, Shook DR, Shimasaki N Cytotoxicity of activated natural killer cells against pediatric solid tumors. Clin. Cancer Res., 2010-06-11;16(15):3901-9. 2010-06-11 [PMID: 20542985] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • Q9BZM5()