UGGT1 Antibody

Western Blot: UGGT1 Antibody [NBP3-33082] - Western blot analysis of UGGT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-33082, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat liver tissue lysate Lane 2: HL-60 cell lysate Lane 3: Mouse liver tissue lysate

Immunohistochemistry: UGGT1 Antibody [NBP3-33082] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-UGGT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33082, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

UGGT1 Antibody Summary

• Description: Novus Biologicals Rabbit UGGT1 Antibody (NBP3-33082) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein within human UGGT1 aa 1350-1555. (Uniprot: Q9NYU2)
• Localization: Endoplasmic reticulum.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UGGT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 177 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.2% BSA and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UGGT1 Antibody

• FLJ21763
• GT
• HUGT1
• UDP--Glc:glycoprotein glucosyltransferase
• UDP-glucose ceramide glucosyltransferase-like 1
• UDP-glucose glycoprotein glucosyltransferase 1
• UGCGL1
• UGGT
• UGT1
• UGTR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UGGT1