UEV2 Antibody - BSA Free

Western Blot: UEV2 Antibody - BSA Free [NBP3-47078] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (UEV2 antibody) at dilution of 1:1500

Immunohistochemistry: UEV2 Antibody - BSA Free [NBP3-47078] - Immunohistochemistry of paraffin-embedded human colon cancer using (UEV2 antibody) at dilution of 1:100

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UEV2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UEV2 Antibody - BSA Free (NBP3-47078) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ubiquitin-conjugating enzyme E2 variant 2. (Uniprot# Q15819)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2V2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Application Notes: Theoretical Molecular Weight is 16-20 kDa

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for UEV2 Antibody - BSA Free

• 1 alpha
• 25-dihydroxyvitamin D3-inducible
• DDVIT1
• DDVit-1
• EDAF-1
• EDPF1
• EDPF-1DDVit 1
• enterocyte differentiation promoting factor
• Enterocyte differentiation-associated factor 1
• enterocyte differentiation-associated factor EDAF-1
• Enterocyte differentiation-promoting factor 1
• methyl methanesulfonate sensitive 2, S. cerevisiae, homolog of
• MMS2
• MMS2MMS2 homolog
• UBE2V2
• ubiquitin-conjugating enzyme E2 variant 2
• Uev2
• UEV-2
• Vitamin D3-inducible protein

Background

The differentiation and activation of monocytes (MO) and monocytic cells is modulated by Vitamin D3. Recently, using mRNA Differential Display technology, Fritsche J, et al have identified a gene which is induced in freshly isolated human blood MO that are cultured for 4 hours with Vitamin D3. This cDNA encodes a putative 16.5 kD protein. The product of this gene has structural homology with yeast MMS2P and human CROC-1, which defines a structurally and functionally conserved UBC-like protein family. This protein was called UEV2. The yeast UEV protein is required together with UBC13 for RAD6/RAD18-dependent postreplicative DNA repair in yeast. These proteins also play a central role in the assembly of K63-linked polyubiquitin chains UEV/UBC complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and IkBa kinase activation. Recent crystal structure analysis provides direct evidence that the UEV2 heterodimer is necessary for DNA repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2V2