UCP3 Antibody

Product Discontinued

UCP3 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 280-320 of Human UCP3.
• Specificity: UCP3 (R308) pAb detects endogenous levels of UCP3 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UCP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of UCP3 (R308) pAb in extracts from Jurkat cells
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (approx. pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UCP3 Antibody

• mitochondrial uncoupling protein 3
• SLC25A9Solute carrier family 25 member 9
• UCP 3
• uncoupling protein 3 (mitochondrial, proton carrier)
• Uncoupling protein-3

Background

The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimer that acts as a proton channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism. UCP2 is a structurally related protein that also uncouples mitochondrial respiration. It is more widely expressed in human and mouse tissues, including white adipose tissue and muscle, than is UCP1. UCP2 is thought to play a role in body weight regulation. An additional UCP family member, UCP3, is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UCP3