UBR4 Antibody

Western Blot: UBR4 Antibody [NBP1-28724] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.04 ug/ml.

Immunohistochemistry: UBR4 Antibody [NBP1-28724] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p600 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

UBR4 Antibody Summary

• Description: Novus Biologicals Rabbit UBR4 Antibody (NBP1-28724) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 5133 and 5183 of human retinoblastoma-associated factor 600 using the numbering given in entry NP_065816.1 (GeneID 23352).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBR4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100- 1:500
  • Immunohistochemistry-Paraffin 1:100- 1:500
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBR4 Antibody

• 600 kDa retinoblastoma protein-associated factor
• EC 6.3.2
• EC 6.3.2.-
• KIAA0462E3 ubiquitin-protein ligase UBR4
• KIAA1307zinc finger, UBR1 type 1
• N-recognin-4
• p600
• RBAF600FLJ41863
• Retinoblastoma-associated factor of 600 kDa
• ubiquitin protein ligase E3 component n-recognin 4
• Zinc finger UBR1-type protein 1
• ZUBR1retinoblastoma-associated factor 600-like protein

Background

p600 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, p600 forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Additionally p600 regulates integrin-mediated signaling and may play a role in activation of FAK in response to cell-matrix interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBR4
• Entrez:
  • 23352(Human)
• Uniprot:
  • NP_065816.1(Human)