Recombinant Human Ubiquitin His Protein

Western Blot: Ubiquitin Protein [NBP1-42465] - The anti-Ubiquitin antibody NB300-129 was used to detect this protein on a western blot. 500ng of protein were loaded on the gel and then detected with NB300-129.

SDS-Page: Ubiquitin Protein [NBP1-42465] - This RPS27a Human Recombinant protein was tested on a 4-20% SDS-PAGE gel with 5ug of protein loaded. The purity of the protein is >95%.

Product Discontinued

Recombinant Human Ubiquitin His Protein Summary

• Description: Recombinant ubiquitin protein with an N-terminal His tag and corresponding to amino acids 1-156.

  Source: E. coli

  (Accession #: P62979)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: RPS27A
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
  • Western Blot
• Application Notes: This protein is useful as a positive control in Western Blotting and SDS-PAGE. This protein has been tested using NB300-129 for detection on a Western Blot.
• Theoretical MW: 20.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 25 mM Tris pH 8.0, 250 mM NaCl, 8 M Urea, 1 mM DTT
• Preservative: N/A
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Ubiquitin His Protein

• RPS27A
• UBA52
• UBB ubiquitin B
• UBB
• UBC
• Ubiquitin

Background

Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. This protein is encoded by the gene RPS27A, which also encodes for an N-terminal ubiquitin fusion partner. It contains C4-type zinc finger domains and is located in the cytoplasm. Human RPS27A with an N-terminal His tag expressed in E. coli and purified by conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RPS27A