Ubiquitin Antibody (1001C) [Unconjugated] - K48 Linkage

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with MG132. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Ubiquitin K48 Linkage Monoclonal Antibody ...read more
25 ng of each linkage of recombinant diubiquitin was run on a 10-20% SDS-PAGE gel prior to blotting on PVDF membrane. Western blots were developed using anti-K48 mAb (A-101, upper panel) or anti-ubiquitin mAb (MAB701, ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, ICC/IF
Clone
1001C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Ubiquitin Antibody (1001C) [Unconjugated] - K48 Linkage Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
K48-linkage from human ubiquitin
Accession # P0CG47
Specificity
This antibody detects endogenous, human proteins containing K48-linked polyubiquitin chains in Western blots. This antibody detects purified, recombinant K48-linked polyubiquitin chains, but has no cross-reactivity to monoubiquitin or polyubiquitin of other linkages
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RPS27A
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
A-101 in the following applications:

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, -20 °C as supplied.
  • 3 months, -20 °C under sterile conditions after opening.
Buffer
0.5 mg/mL in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide
Preservative
0.09% Sodium Azide

Notes

Not for use in humans

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ubiquitin Antibody (1001C) [Unconjugated] - K48 Linkage

  • RPS27A
  • UBA52
  • UBB ubiquitin B
  • UBB
  • UBC
  • Ubiquitin

Background

Polyubiquitin chains are composed of ubiquitin monomers that are covalently linked through isopeptide bonds (other than linear, or “Met1-linked” polyubiquitin). Isopeptides are formed between a lysine residue of one Ubiquitin molecule and the C­terminal glycine residue of another Ubiquitin molecule. Seven of the seventy-six amino acids in ubiquitin are lysine residues that can participate in polyubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes. K48-linked polyubiquitin chains attached to substrate proteins often serve as a recognition sequence for targeting and destruction of the substrate by the 26S Proteasome. This antibody detects the K48 linkage. It does not detect monoubiquitin or ubiquitin liked via any other lysine residue. Reactivity across all species is anticipated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ubiquitin Antibody (A-101)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Western Blot.


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Video Protocols

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Bioinformatics

Gene Symbol RPS27A
Uniprot