UBE4B Antibody - BSA Free

Western Blot: UBE4B Antibody [NBP1-32737] - (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE diluted at 1:10000

Immunocytochemistry/Immunofluorescence: UBE4B Antibody [NBP1-32737] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: UBE4B Antibody [NBP1-32737] - CA922 xenograft, using UBE4B antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UBE4B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UBE4B Antibody - BSA Free (NBP1-32737) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human UBE4B. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE4B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 146 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UBE4B Antibody - BSA Free

• E4
• homologous to yeast UFD2
• Homozygously deleted in neuroblastoma 1
• homozygously deleted in neuroblastoma-1
• KIAA0684HDNB1
• UBE4B
• ubiquitin conjugation factor E4 B
• Ubiquitin fusion degradation protein 2
• ubiquitination factor E4B (homologous to yeast UFD2)
• ubiquitination factor E4B (UFD2 homolog, yeast)
• ubiquitin-fusion degradation protein 2
• UBOX3
• UFD2
• UFD2a
• UFD2UFD2A

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UBE4B