UBE4B Antibody

Images

 
Western Blot: UBE4B Antibody [NBP1-32737] - (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE diluted at 1:10000
Immunocytochemistry/ Immunofluorescence: UBE4B Antibody [NBP1-32737] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: UBE4B Antibody [NBP1-32737] - Paraffin-embedded CA922 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

UBE4B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human UBE4B. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE4B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBE4B Antibody

  • E4
  • homologous to yeast UFD2
  • Homozygously deleted in neuroblastoma 1
  • homozygously deleted in neuroblastoma-1
  • KIAA0684HDNB1
  • ubiquitin conjugation factor E4 B
  • Ubiquitin fusion degradation protein 2
  • ubiquitination factor E4B (homologous to yeast UFD2)
  • ubiquitination factor E4B (UFD2 homolog, yeast)
  • ubiquitin-fusion degradation protein 2
  • UBOX3
  • UFD2UFD2A

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol UBE4B