UBE2Q2 Antibody

Western Blot: UBE2Q2 Antibody [NBP1-49694] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-UBE2Q2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.

• Reactivity: Hu, Mu, Bv, Rb
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

UBE2Q2 Antibody Summary

• Description: Novus Biologicals Rabbit UBE2Q2 Antibody (NBP1-49694) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 325 and 375 of human Ubiquitin-Conjugating Enzyme E2Q Family Member 2 using the numbering given in entry NP_775740.1 (GeneID 92912).
• Predicted Species: Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2Q2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBE2Q2 Antibody

• DKFZp762C143
• EC 6.3.2.19
• LOC92912
• UBE2Q2
• Ubiquitin carrier protein Q2
• ubiquitin-conjugating enzyme E2 Q2
• ubiquitin-conjugating enzyme E2Q (putative) 2
• ubiquitin-conjugating enzyme E2Q family member 2
• Ubiquitin-protein ligase Q2

Background

The ubiquitination pathway is known to regulate critical control points in multiple cellular processes by targeting proteins for activation, degradation, or localization at specific intracellular sites. The pathway involves an enzymatic cascade in which the first step activates ubiquitin by forming a high energy thiolester intermediate to ubiquitin activating enzyme (E1) that is subsequently transferred to members of an E2 ubiquitin conjugating enzyme superfamily. The E2 ubiquitin conjugating 45 enzymes function together with a larger superfamily of E3 ubiquitin ligases to transfer activated ubiquitin to specific cellular proteins. The existence of E2 and E3 superfamilies reflects the specificity of each E2-E3 cognate pair for a specific group of cellular substrates. UBE2Q2 is a potentially unique member of the ubiquitin conjugating enzyme superfamily that includes at least 17 phenotypically different subfamilies, many of which contain isozymes. Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2Q2