MSS4 Antibody - BSA Free

Western Blot: MSS4 Antibody [NBP2-93502] - Analysis of extracts of various cell lines, using MSS4 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

Immunocytochemistry/ Immunofluorescence: MSS4 Antibody - BSA Free [NBP2-93502] - Immunofluorescence analysis of U2OS cells using MSS4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MSS4 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-470 of human PIP5K1B (NP_003549.1). NVLPRSMRMHFTYDLKGSTYKRRASRKEREKSNPTFKDLDFLQDMHEGLYFDTETYNALMKTLQRDCRVLESFKIMDYSLLLGIHFLDHSLKEKEEETPQNVPDAKRTGMQKVLYSTAMESIQGPGKSGDGIITENPDTMGGIPAKSHRGEKLLLFMGIIDILQSYRLMKKLEHSWKALVYDGDTVSVHRPSFYADRFLKFMNSRVFKKIQALKASPSKKRCNSIAALKATSQEIVSSISQEWKDEKRDLLTEGQSFSSLDEEALGSRHRPDLVPSTPSLF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIP5K1B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MSS4 Antibody - BSA Free

• EC 2.7.1
• MSS4Protein STM-7
• Phosphatidylinositol-4-phosphate 5-kinase type I beta
• phosphatidylinositol-4-phosphate 5-kinase type-1 beta
• phosphatidylinositol-4-phosphate 5-kinase, type I, beta
• PIP5K1-beta
• PIP5KIbeta
• PtdIns(4)P-5-kinase 1 beta
• STM7EC 2.7.1.68
• Type I phosphatidylinositol-4-phosphate 5-kinase beta

Background

MSS4, also known by its gene name PIP5K1B, has a 543 amino acid long isoform that is 61 kDa and a 549 amino acid isoform that is approximately 62 kDa. MSS4 plays a role in platelet adhesion, actin filament organization and the biosynthesis of PIP2. Current research on MSS4 is being conducted in relation to several diseases and disorders including kidney disease, schizophrenia, neuronitis and Friedreich's ataxia. MSS4 has also been shown to have interactions with CLIC1, BTK, MCRS1 and PIP5KL1 in pathways such as the RAC1 signaling pathway, CD28 signaling, Phosphatidylinositol signaling and the metabolism of lipids and lipoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIP5K1B