UBA3 Antibody

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Western Blot: UBA3 Antibody [NBP2-20777] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:3000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

UBA3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human UBA3. The exact sequence is proprietary.
Predicted Species
Mouse (99%), Rat (99%), Rhesus Macaque (100%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBA3 Antibody

  • DKFZp566J164
  • EC 6.3.2
  • EC 6.3.2.-
  • hUBA3
  • MGC22384
  • NEDD8-activating enzyme E1 catalytic subunit
  • NEDD8-activating enzyme E1C
  • Nedd8-activating enzyme hUba3
  • UBA3
  • UBA3, ubiquitin-activating enzyme E1 homolog
  • UBE1C
  • Ubiquitin-activating enzyme 3
  • ubiquitin-activating enzyme E1C (homologous to yeast UBA3)
  • Ubiquitin-activating enzyme E1C
  • ubiquitin-like modifier activating enzyme 3
  • Ubiquitin-like modifier-activating enzyme 3
  • yeast)

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol UBA3
Entrez
Uniprot