UBA3 Antibody - BSA Free

Western Blot: UBA3 Antibody [NBP2-20777] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:3000.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UBA3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UBA3 Antibody - BSA Free (NBP2-20777) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UBA3. The exact sequence is proprietary.
• Predicted Species: Mouse (99%), Rat (99%), Rhesus Macaque (100%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBA3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UBA3 Antibody - BSA Free

• DKFZp566J164
• EC 6.3.2
• EC 6.3.2.-
• hUBA3
• MGC22384
• NEDD8-activating enzyme E1 catalytic subunit
• NEDD8-activating enzyme E1C
• Nedd8-activating enzyme hUba3
• UBA3
• UBA3, ubiquitin-activating enzyme E1 homolog
• UBE1C
• Ubiquitin-activating enzyme 3
• ubiquitin-activating enzyme E1C (homologous to yeast UBA3)
• Ubiquitin-activating enzyme E1C
• ubiquitin-like modifier activating enzyme 3
• Ubiquitin-like modifier-activating enzyme 3
• yeast)

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UBA3
• Uniprot:
  • Q8TBC4(Human)