U2AF35 Antibody

Images

 
Western Blot: U2AF35 Antibody [NBP1-32515] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: U2AF35 Antibody [NBP1-32515] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-32515] - Paraffin-embedded mouse intestine. U2AF35 antibody diluted at 1:1000.
Immunoprecipitation: U2AF35 Antibody [NBP1-32515] - U2AF35 protein from 293T whole cell extracts using 5 ug of U2AF35 antibody. Western blot analysis was performed using U2AF35 antibody. EasyBlot anti-Rabbit IgG was ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.55 mg/ml

Order Details

U2AF35 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human U2AF35. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rat (99%), Xenopus (97%), Bovine (98%), Chicken (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
U2AF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Application Notes
WB: Detects an approx. 37 kDa protein.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.55 mg/ml
Purity
Immunogen affinity purified

Alternate Names for U2AF35 Antibody

  • FP793
  • human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712
  • RN
  • RNU2AF1,35-KD subunit
  • U2 auxiliary factor 35 kDa subunit
  • U2 small nuclear RNA auxiliary factor 1
  • U2 small nuclear RNA auxillary factor 1
  • U2 snRNP auxiliary factor small subunit

Background

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol U2AF1
Entrez
Uniprot