U2AF35 Antibody

Western Blot: U2AF35 Antibody [NBP1-32515] - Sample(30ug whole cell lysate)A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-32515] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-32515] - Paraffin-embedded mouse intestine. U2AF35 antibody diluted at 1:1000.

Immunoprecipitation: U2AF35 Antibody [NBP1-32515] - U2AF35 protein from 293T whole cell extracts using 5 ug of U2AF35 antibody. Western blot analysis was performed using U2AF35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Xp
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

U2AF35 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human U2AF35. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (99%), Chicken (98%), Xenopus (97%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: U2AF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Application Notes: WB: Detects an approx. 37 kDa protein.
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (97%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7)
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for U2AF35 Antibody

• FP793
• human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712
• RN
• RNU2AF1,35-KD subunit
• U2 auxiliary factor 35 kDa subunit
• U2 small nuclear RNA auxiliary factor 1
• U2 small nuclear RNA auxillary factor 1
• U2 snRNP auxiliary factor small subunit

Background

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: U2AF1
• Entrez:
  • 512680(Bovine)
  • 7307(Human)
  • 108121(Mouse)
• Uniprot:
  • Q01081(Human)