U2AF35 Antibody

Images

 
Independent Antibodies: Western Blot: U2AF35 Antibody [NBP1-19120] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. U2AF35 was also immunoprecipitated by rabbit anti-U2AF35 antibody NBP1-19121.
Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-19120] - Section of human lung carcinoma (left) and mouse CT26 colon carcinoma (right).

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

U2AF35 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 140 and 190 of human U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit using the numbering given in entry NP_006749.1 (GeneID 7307).
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
U2AF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publication using NBP1-19120.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for U2AF35 Antibody

  • FP793
  • human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712
  • RN
  • RNU2AF1,35-KD subunit
  • U2 auxiliary factor 35 kDa subunit
  • U2 small nuclear RNA auxiliary factor 1
  • U2 small nuclear RNA auxillary factor 1
  • U2 snRNP auxiliary factor small subunit

Background

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for U2AF35 Antibody (NBP1-19120)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol U2AF1
Entrez
Uniprot