U2AF35 Antibody

Western Blot: U2AF35 Antibody [NBP1-19120] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. U2AF35 was also immunoprecipitated by rabbit anti-U2AF35 antibody NBP1-19121.

Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-19120] - Section of human lung carcinoma (left) and mouse CT26 colon carcinoma (right).

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

U2AF35 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 140 and 190 of human U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit using the numbering given in entry NP_006749.1 (GeneID 7307).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: U2AF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for U2AF35 Antibody

• FP793
• human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712
• RN
• RNU2AF1,35-KD subunit
• U2 auxiliary factor 35 kDa subunit
• U2 small nuclear RNA auxiliary factor 1
• U2 small nuclear RNA auxillary factor 1
• U2 snRNP auxiliary factor small subunit

Background

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for U2AF35 Antibody (NBP1-19120)(1)

Publication using NBP1-19120

• Wang C, Wilson-Berry L, Schedl T, Hansen D. TEG-1 CD2BP2 regulates stem cell proliferation and sex determination in the C. elegans germ line and physically interacts with the UAF-1 U2AF65 splicing factor. Developmental Dynamics: An Official Publication of the American Association of Anatomists. 2012-01-13 [PMID: 22275078]

Bioinformatics

• Gene Symbol: U2AF1
• Entrez:
  • 7307(Human)
  • 108121(Mouse)
• Uniprot:
  • NP_006749.1(Human)