TXNIP Antibody - BSA Free

Western Blot: TXNIP Antibody [NBP2-27095] - Analysis of TXNIP in Raji cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu, Rt, Pm, Pm, Po, Pm-Or
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TXNIP Antibody - BSA Free Summary

• Immunogen: A portion of amino acids 100-150 of human TxNIP was used as the immunogen.
• Predicted Species: Porcine (96%), Orangutan (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TXNIP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4ug/ml

Reactivity Notes

Rat reactivity reported in scientific literature (Singatulina et al). Use in Mouse reported in scientific literature (PMID:32164364).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for TXNIP Antibody - BSA Free

• HHCPA78
• THIF
• thioredoxin binding protein 2
• thioredoxin interacting protein
• Thioredoxin-binding protein 2
• thioredoxin-interacting protein
• upregulated by 1,25-dihydroxyvitamin D-3
• VDUP1EST01027
• Vitamin D3 up-regulated protein 1

Background

Thioredoxin-interacting protein belongs to the arrestin family. It acts as an oxidative stress mediator by inhibiting thioredoxin activity or by inhibiting thioredoxin activity or by limiting its bioavailability. It functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. It is required for the maturation of natural killer cells. Recently reported, TXNIP deficiency led to the impairment of NLRP3 inflammasome activation to innate immune responses and reduced secretion of interleukin IL-1Beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TXNIP Antibody (NBP2-27095)(4)

Publications using NBP2-27095

• Singatulina AS, Hamon L, Sukhanova MV et al. Evaluation of the Effect of Carvedilol on the Thioredoxin Pathway in H9C2 Rat Cardiomyocytes Thesis (IP, WB, Rat, Monkery)
• Karmi O, Sohn YS, Zandalinas SI et al. Disrupting CISD2 function in cancer cells primarily impacts mitochondrial labile iron levels and triggers TXNIP expression Free Radical Biology and Medicine 2021-11-01 [PMID: 34547371] (Western Blot, Human)
• Lim Jo, Shin Nr, Seo Ys et Al. Silibinin Attenuates Silica Dioxide Nanoparticles-Induced Inflammation by Suppressing TXNIP/MAPKs/AP-1 Signaling Cells 2020-03-10 [PMID: 32164364] (IHC-P, IF/IHC, WB, Mouse)
• Lee H, Lee S, Bae H, Kang HS, Kim SJ. Genome-wide identification of target genes for miR-204 and miR-211 identifies their proliferation stimulatory role in breast cancer cells. Nature.com. 2016-04-28 [PMID: 27121770] (WB, Human)

Bioinformatics

• Gene Symbol: TXNIP
• Uniprot:
  • Q9H3M7(Human)