TXNDC5 Antibody

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human TXNDC5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4840) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TXNDC5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TXNDC5 Antibody Summary

• Immunogen: E. coli-derived recombinant human TXNDC5
  Glu110-Leu432
  Accession # Q8NBS9
• Specificity: Detects endogenous human TXNDC5 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TXNDC5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TXNDC5 Antibody

• EndoPDI
• ERp46
• FLJ90810
• HCC-2
• member 15
• MGC3178
• PDIA15
• thioredoxin domain containing 5 (endoplasmic reticulum)
• thioredoxin domain containing 5
• thioredoxin domain-containing protein 5
• thioredoxin related protein
• TLP46
• TXNDC5

Background

TXNDC5 (Thioredoxin (TRX) domain-contain protein 5; also ERp46 and EndoPDI) is a 50‑55 kDa member of the disulfide isomerase family of enzymes. It is expressed in hepatocytes, and tumor plus hypoxic endothelium. TXNDC5 demonstrates thioreductase (disulfide bond reduction) activity in the ER. Mature human TXNDC5 is 400 amino acids (aa) in length and migrates anomalously in SDS-PAGE. It contains three TRX domains (aa 36‑428) and a short ER retention motif (aa 429‑432). There are three potential splice variants. One shows an alternate start site at Met109, while two others show a 16 and 45 aa substitution for the N-terminal 88 amino acids. Over aa 110‑432, human TXNDC5 is 88% aa identical to mouse TXNDC5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for TXNDC5 Antibody (AF4840) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/08/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 19925638
• Species: Human

Bioinformatics

• Gene Symbol: TXNDC5
• Uniprot:
  • Q8NBS9(Human)