Tubulin Beta 4 Antibody - BSA Free

Western Blot: Tubulin Beta 4 Antibody [NBP1-32205] - Sample(30 ug of whole cell lysate) A:293T7. 5% SDS PAGE, antibody diluted at 1:1000.

Western Blot: Tubulin Beta 4 Antibody [NBP1-32205] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Tubulin Beta 4 Antibody [NBP1-32205] - Paraformaldehyde-fixed U2OS, using beta Tubulin 4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

Tubulin Beta 4 antibody detects Tubulin Beta 4 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tubulin Beta 4 stained by Tubulin Beta 4 antibody (NBP1-32205) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Bv, Pm, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Tubulin Beta 4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Tubulin Beta 4 Antibody - BSA Free (NBP1-32205) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Tubulin Beta 4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Tubulin Beta 4. The exact sequence is proprietary.
• Predicted Species: Xenopus (96%), Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBB4A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: IHC usage reported in scientific literature (PMID: 24624341).
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Tubulin Beta 4 Antibody - BSA Free

• TUBB4
• TUBB4A
• TUBB5beta-5
• Tubulin 5 beta
• Tubulin Beta 4
• tubulin beta-4 chain
• tubulin, beta 4
• tubulin, beta, 5

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Tubulin Beta 4 Antibody (NBP1-32205)(1)

Publication using NBP1-32205

• Xu Weiling, Janocha Allison J, Leahy Rachel A et al. A novel method for pulmonary research: assessment of bioenergetic function at the air-liquid interface. Redox Biol. 2014-01-16 [PMID: 24624341] (IF/IHC)

Bioinformatics

• Gene Symbol: TUBB4A