Tubulin Beta 4 Antibody (SR2377)

Immunocytochemistry/ Immunofluorescence: Tubulin Beta 4 Antibody (SR2377) [NBP3-27688] - Immunocytochemistry analysis of Tubulin Beta 4 (green) in Hela using NBP3-27688 ,and DAPI (blue)

Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (SR2377) [NBP3-27688] - Immunohistochemistry analysis of paraffin-embedded Human brain. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western Blot: Tubulin Beta 4 Antibody (SR2377) [NBP3-27688] - Western blot analysis of mouse brain lysates

Western Blot: Tubulin Beta 4 Antibody (SR2377) [NBP3-27688] - Western blot analysis in K562, rat Brain, C6, Hela lysates

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: SR2377
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Tubulin Beta 4 Antibody (SR2377) Summary

• Description: Novus Biologicals Rabbit Tubulin Beta 4 Antibody (SR2377) (NBP3-27688) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Tubulin Beta 4 (Uniprot #: P04350)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TUBB4A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Tubulin Beta 4 Antibody (SR2377)

• TUBB4
• TUBB4A
• TUBB5beta-5
• Tubulin 5 beta
• Tubulin Beta 4
• tubulin beta-4 chain
• tubulin, beta 4
• tubulin, beta, 5

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB4A