TTK Antibody (4G9) - Azide and BSA Free

Western Blot: TTK Antibody (4G9) [H00007272-M02] - TTK monoclonal antibody (M02), clone 4G9 Analysis of TTK expression in Jurkat.

ELISA: TTK Antibody (4G9) [H00007272-M02] - Detection limit for recombinant GST tagged TTK is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TTK Antibody (4G9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TTK (AAH00633, 1 a.a. ~ 176 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVERGAVP
• Specificity: TTK (4G9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TTK
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA. ICC/IF usage was reported in scientific literature.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TTK Antibody (4G9) - Azide and BSA Free

• CT96
• dual specificity protein kinase TTK
• EC 2.7.12.1
• ESK
• FLJ38280
• monopolar spindle 1-like 1
• Mps1
• MPS1L1
• MPS1L1PYTcancer/testis antigen 96
• phosphotyrosine picked threonine kinase
• Phosphotyrosine picked threonine-protein kinase
• PYT
• TTK protein kinase
• TTK

Background

This 95 kDa protein phosphorylates other proteins on serine, threonine, and tyrosine. It has been thought to be associated with cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TTK Antibody (H00007272-M02)(4)

Publications using H00007272-M02

• Sawant DB, Majumder S, Perkins JL et al. Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes Centrin 2 function. Mol. Biol. Cell 2015-09-09 [PMID: 26354417] (ICC/IF, Human)
• Majumder S, Fisk HA. VDAC3 and Mps1 negatively regulate ciliogenesis Cell Cycle 2013-03-01 [PMID: 23388454] (ICC/IF, Human)
• Majumder S, Slabodnick M, Pike A et al. VDAC3 regulates centriole assembly by targeting Mps1 to centrosomes Cell Cycle 2012-08-30 [PMID: 22935710] (WB, ICC/IF, Human)
• Kasbek C, Yang CH, Fisk HA. Antizyme restrains centrosome amplification by regulating the accumulation of Mps1 at centrosomes. Mol Biol Cell. 2010-11-15 [PMID: 20861309] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: TTK
• Entrez:
  • 7272(Human)
• OMIM:
  • 604092(Human)
• Uniprot:
  • AAH00633(Human)