TTF-1 / NKX2-1 Antibody - BSA Free

Western Blot: TTF-1 / NKX2-1 Antibody [NBP2-41160] - Analysis of NKX2-1 expression in human lung tissue lysate with NKX2-1 antibody at (A) 0.25 and (B) 0.5 ug/ml.

Immunohistochemistry: TTF-1 / NKX2-1 Antibody [NBP2-41160] - Immunohistochemistry of NKX2-1 in human lung tissue with NKX2-1 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: TTF-1 / NKX2-1 Antibody - BSA Free [NBP2-41160] - Immunofluorescence of TTF-1 / NKX2-1 in human lung tissue with TTF-1 / NKX2-1 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TTF-1 / NKX2-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TTF-1/ NKX2-1 Antibody - BSA Free (NBP2-41160) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NKX2-1. The immunogen is located within the first 50 amino acids of NKX2-1. Amino Acid Squence: LSPLEESYKKVGMEGG
• Specificity: NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NKX2-1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Western Blot 0.25 - 0.5 ug/mL
• Theoretical MW: 41, 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TTF-1 / NKX2-1 Antibody - BSA Free

• BCH
• benign chorea
• BHC
• Homeobox protein NK-2 homolog A
• homeobox protein Nkx-2.1
• NK2 homeobox 1
• NK-2 homolog A
• NKX2.1
• NKX2-1
• NKX2A
• NKX2ANKX2.1
• T/EBP
• Thyroid nuclear factor 1
• Thyroid transcription factor 1BHC
• TITF1
• TITF1TEBP
• TTF1
• TTF-1
• TTF1BCH
• TTF-1NK-2

Background

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NKX2-1