TSPAN8/TM4SF3 Antibody (657909)

CMT‑93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 Monoclonal Antibody (Catalog # MAB6524, filled histo­gram) or isotype control antibody (Catalog # MAB0061, open histo­gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

TSPAN8 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse TSPAN8 Monoclonal Antibody (Catalog # MAB6524) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Northern­Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to membranes of epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 657909
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TSPAN8/TM4SF3 Antibody (657909) Summary

• Immunogen: CHO Chinese hamster ovary cell line transfected with mouse TSPAN8
• Specificity: Detects mouse TSPAN8 transfectants but not the parental cell line in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: TSPAN8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN8/TM4SF3 Antibody (657909)

• CO-029
• tetraspanin 8
• TM4SF3
• TM4SF3tspan-8
• Transmembrane 4 superfamily member 3tetraspanin-8
• TSPAN8
• Tspan-8
• Tumor-associated antigen CO-029

Background

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 (tetraspanin) superfamily. It is a cell surface 27 - 34 kDa protein with intracellular N- and C-termini, 4 transmembrane segments and a large extracellular loop (LEL) domain from aa 106-206. TSPAN8 is involved in integrin-mediated cell motility, metastasis, cell proliferation and differentiation, and is over-expressed in carcinomas including hepatocellular carcinoma and colon cancer. Polymorphisms have variably been associated with type II diabetes, bipolar disorder and schizophrenia. Human TSPAN8 shares 70% aa sequence identity with mouse and rat TSPAN8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSPAN8/TM4SF3 Antibody (MAB6524)(1)

Publication using MAB6524

• Takahiro Fukaishi, Yuko Nakagawa, Ayako Fukunaka, Takashi Sato, Akemi Hara, Keiko Nakao et al. Characterisation of Ppy-lineage cells clarifies the functional heterogeneity of pancreatic beta cells in mice Diabetologia 2021-12-01 [PMID: 34498099]

Bioinformatics

• Gene Symbol: TSPAN8