TSPAN8/TM4SF3 Antibody (657909) [Phycoerythrin]

CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 PE-conjugated Monoclonal Antibody (Catalog # FAB6524P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 657909
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

TSPAN8/TM4SF3 Antibody (657909) [Phycoerythrin] Summary

• Immunogen: CHO Chinese hamster ovary cell line transfected with mouse TSPAN8
  Accession # Q8R3G9
• Specificity: Detects mouse TSPAN8 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: TSPAN8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN8/TM4SF3 Antibody (657909) [Phycoerythrin]

• CO-029
• tetraspanin 8
• TM4SF3
• TM4SF3tspan-8
• Transmembrane 4 superfamily member 3tetraspanin-8
• TSPAN8
• Tspan-8
• Tumor-associated antigen CO-029

Background

TSPAN8 (Tetraspanin-8), also known as TM4SF3 and CO-029, is a 27-33 kDa member of the tetraspanin family of proteins. As evidenced by its name, it is a 4-transmembrane (glyco)protein that is found on/in multiple tumor types as well as smooth and skeletal muscle, endothelium and hematopoietic progenitor cells. All 4-transmembrane proteins are not tetraspanins. Tetraspanins are characterized by the presence of a very short intracellular C- and N-terminus, and a very long extracellular (or LEL) domain linking the 3rd and 4th transmembrane segments. On TSPAN8 this domain is 98 amino acids (aa) in length and represents 42% of the entire length of mouse TSPAN8 (or 235 aa). TSPAN8, like other tetraspanins, forms the backbone of a network that allows molecule interactions on (or in) plasma membranes. This ability is dependent upon molecule palmitoylation and creates a microdomain (or TEM) that is distinct from typical membrane rafts, the latter of which is characterized by the presence of GPI-linked proteins and the molecule caveolin. Within this TEM, TSPAN8 may form homodimers, and is known to interact with multiple molecule types, including CD151, CD81, CD9, CD13, EpCAM, EWI-F, MMP-9, Meprin-beta , Rictor, TACE and the integrins alpha 3(CD49c) beta 1, alpha 6 beta 4 and alpha 4(CD49d) beta 1. Such interactions appear to positively or negatively modulate individual molecule activity. These interactions are generally described within the context of exosomes, 30-100 nm membrane-bound vesicles that are released from multiple cell types. TSPAN8-containing exosomes are reported to either facilitate cell motility or act as an antiapoptotic agent for target cells. Over aa 106-203 constituting the LEL, mouse TSPAN8 shares 58% and 73% aa sequence identity with human and rat TSPAN8, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSPAN8/TM4SF3 Antibody (FAB6524P)(2)

Publications using FAB6524P

• J Yang, F Guo, HS Chin, GB Chen, CH Ang, Q Lin, W Hong, NY Fu Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins Cell Reports, 2023-01-31;42(2):112065. 2023-01-31 [PMID: 36724073] (Flow Cytometry, Human)
• NY Fu, AC Rios, B Pal, CW Law, P Jamieson, R Liu, F Vaillant, F Jackling, KH Liu, GK Smyth, GJ Lindeman, ME Ritchie, JE Visvader Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive Nat. Cell Biol, 2017-02-13;19(3):164-176. 2017-02-13 [PMID: 28192422] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TSPAN8
• Uniprot:
  • Q8R3G9()