TSPAN8/TM4SF3 Antibody (657909) [Allophycocyanin]

CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 APC-conjugated Monoclonal Antibody (Catalog # FAB6524A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 657909
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

TSPAN8/TM4SF3 Antibody (657909) [Allophycocyanin] Summary

• Immunogen: CHO Chinese hamster ovary cell line transfected with mouse TSPAN8
• Specificity: Detects mouse TSPAN8 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: TSPAN8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN8/TM4SF3 Antibody (657909) [Allophycocyanin]

• CO-029
• tetraspanin 8
• TM4SF3
• TM4SF3tspan-8
• Transmembrane 4 superfamily member 3tetraspanin-8
• TSPAN8
• Tspan-8
• Tumor-associated antigen CO-029

Background

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 (tetraspanin) superfamily. It is a cell surface 27 - 34 kDa protein with intracellular N- and C-termini, 4 transmembrane segments and a large extracellular loop (LEL) domain from aa 106-206. TSPAN8 is involved in integrin-mediated cell motility, metastasis, cell proliferation and differentiation, and is over-expressed in carcinomas including hepatocellular carcinoma and colon cancer. Polymorphisms have variably been associated with type II diabetes, bipolar disorder and schizophrenia. Human TSPAN8 shares 70% aa sequence identity with mouse and rat TSPAN8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSPAN8/TM4SF3 Antibody (FAB6524A)(3)

Publications using FAB6524A

• Klein, F;Veiga-Villauriz, C;Börsch, A;Maio, S;Palmer, S;Dhalla, F;Handel, AE;Zuklys, S;Calvo-Asensio, I;Musette, L;Deadman, ME;White, AJ;Lucas, B;Anderson, G;Holländer, GA; Combined multidimensional single-cell protein and RNA profiling dissects the cellular and functional heterogeneity of thymic epithelial cells Nature communications 2023-07-10 [PMID: 37429879] (Flow Cytometry, Transgenic Mouse)
• B Kumar, AK Adebayo, M Prasad, ML Capitano, R Wang, P Bhat-Naksh, M Anjanappa, E Simpson, D Chen, Y Liu, JM Schilder, AB Colter, C Maguire, CJ Temm, G Sandusky, EH Doud, AB Wijeratne, AL Mosley, HE Broxmeyer, H Nakshatri Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity Science Advances, 2022-01-12;8(2):eabh3375. 1/12/2022 [PMID: 35020422]
• NY Fu, AC Rios, B Pal, CW Law, P Jamieson, R Liu, F Vaillant, F Jackling, KH Liu, GK Smyth, GJ Lindeman, ME Ritchie, JE Visvader Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive Nat. Cell Biol, 2017-02-13;19(3):164-176. 2017-02-13 [PMID: 28192422] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TSPAN8