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TSPAN7/TM4SF2 Antibody (B2D)

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Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) [NBP2-75971] - Surface staining of CD231 in JURKAT cells with anti-CD231 (B2D) FITC.
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) [NBP2-75971] - Surface staining of CD231 in JURKAT cells with anti-CD231 (B2D) FITC.
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) [NBP2-75971] - Surface staining of CD231 in JURKAT cells with anti-CD231 (B2D) purified, GAM-APC.
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) [NBP2-75971] - Surface staining of CD231 in JURKAT cells with anti-CD231 (B2D) PE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IP
Clone
B2D
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-75971AF350
Alexa Fluor 405
NBP2-75971AF405
Alexa Fluor 488
NBP2-75971AF488
Alexa Fluor 532
NBP2-75971AF532
Alexa Fluor 594
NBP2-75971AF594
Alexa Fluor 647
NBP2-75971AF647
Alexa Fluor 700
NBP2-75971AF700
Alexa Fluor 750
NBP2-75971AF750
Allophycocyanin
NBP2-75971APC
Biotin
NBP2-75971B
DyLight 350
NBP2-75971UV
DyLight 405
NBP2-75971V
DyLight 488
NBP2-75971G
DyLight 550
NBP2-75971R
DyLight 594
NBP2-75971DL594
DyLight 650
NBP2-75971C
DyLight 680
NBP2-75971FR
DyLight 755
NBP2-75971IR
FITC
NBP2-75971F
HRP
NBP2-75971H
Janelia Fluor 549
NBP2-75971JF549
Janelia Fluor 646
NBP2-75971JF646
PE
NBP2-75971PE
PerCP
NBP2-75971PCP
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TSPAN7/TM4SF2 Antibody (B2D) Summary

Immunogen
Human T-ALL cell line THP-6
Specificity
The antibody QBEnd-10 reacts with Class II epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TSPAN7
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for TSPAN7/TM4SF2 Antibody (B2D)

  • A15
  • A15CCG-B7
  • CCG-B7
  • CD231 antigen
  • CD231
  • Cell surface glycoprotein A15
  • DXS1692E
  • Membrane component chromosome X surface marker 1
  • mental retardation, X-linked 58
  • MRX58
  • MXS1
  • MXS1T-cell acute lymphoblastic leukemia associated antigen 1
  • TALLA-1
  • T-cell acute lymphoblastic leukemia-associated antigen 1
  • tetraspanin 7
  • tetraspanin-7
  • TM4SF2
  • TM4SF2b
  • TM4SF2tetraspanin protein
  • transmembrane 4 superfamily 2b
  • Transmembrane 4 superfamily member 2X chromosome, surface marker 1
  • transmembrane protein A15
  • TSPAN7
  • tspan-7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep36502), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: CD81 Antibody (1D6) [NB100-65805] - The TAPA1 Antibody (1D6) was tested in A431 cells at a 1:10 dilution against DyLight 488 (green). Actin was counterstained against Phalloidin 568 (red) and cells were mounted in DAPI Flouromount (blue).
Immunohistochemistry-Paraffin: CD81 Antibody (1D6) [NB100-65805] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain.
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CD63 Antibody
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Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - THP1 whole cell protein was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with anti-CD63 antibody at 2 ug/mL and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - The CD63 (H5C6) antibody was tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 (H5C6) Antibody NBP2-42225R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
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NBP2-21792
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Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.
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Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. ICC/IF image submitted by a verified customer review.
Simple Western: Tyrosine Hydroxylase Antibody [NB300-109] - Simple Western lane view shows a specific band for Tyrosine Hydroxylase in 0.2 mg/mL of PC-12 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Immunohistochemistry-Paraffin: TM4SF1/L6 Antibody [NBP1-76549] - Mouse lung tissue with TM4SF1 antibody at 2 ug/mL.
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Western Blot: RSK2 Antibody [NBP2-20237] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE gel, diluted at 1:2000.
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Western Blot: ARHGEF6 Antibody [NBP1-89124] - Analysis in human cell line HL-60.
Immunocytochemistry/Immunofluorescence: ARHGEF6 Antibody [NBP1-89124] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
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Flow Cytometry: IL1RAPL1 Antibody (3E3D12) [NBP2-52493] - Analysis of Hela cells using IL1RAPL1 mouse mAb (green) and negative control (red).
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Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Antibody
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Immunohistochemistry-Paraffin: Peripherin 2/PRPH2 Antibody [NBP1-86687] - Staining of retina shows strong cytoplasmic positivity in photoreceptors.
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Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.
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IGSF8/CD316
Antibody [Unconjugate
AF3117
Neuro‑2A mouse neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, DU145 human prostate carcinoma cell line, and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for IGSF8/CD316 at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of rat brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IGSF8/CD316 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, CyTOF-ready, ICC

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-75971AF350
Alexa Fluor 405 NBP2-75971AF405
Alexa Fluor 488 NBP2-75971AF488
Alexa Fluor 532 NBP2-75971AF532
Alexa Fluor 594 NBP2-75971AF594
Alexa Fluor 647 NBP2-75971AF647
Alexa Fluor 700 NBP2-75971AF700
Alexa Fluor 750 NBP2-75971AF750
Allophycocyanin NBP2-75971APC
Biotin NBP2-75971B
DyLight 350 NBP2-75971UV
DyLight 405 NBP2-75971V
DyLight 488 NBP2-75971G
DyLight 550 NBP2-75971R
DyLight 594 NBP2-75971DL594
DyLight 650 NBP2-75971C
DyLight 680 NBP2-75971FR
DyLight 755 NBP2-75971IR
FITC NBP2-75971F
HRP NBP2-75971H
Janelia Fluor 549 NBP2-75971JF549
Janelia Fluor 646 NBP2-75971JF646
PE NBP2-75971PE
PerCP NBP2-75971PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol TSPAN7