Neuro‑2A mouse neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117, filled histogram) or isotype control antibody (Catalog # ...read more
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, DU145 human prostate carcinoma cell line, and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
Western blot shows lysates of rat brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) ...read more
Simple Western lane view shows lysates of human cerebellum tissue, human hippocampus tissue, Neuro‑2A mouse neuroblastoma cell line, and rat hippocampus tissue, loaded at 0.2 mg/mL. A specific band was ...read more
IGSF8/CD316 was detected in immersion fixed Neuro‑2A mouse neuroblastoma cell line using Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) at 10 µg/mL for 3 ...read more
Detects mouse IGSF8/CD316 in direct ELISAs. Detects human, mouse, and rat IGSF8/CD316 in Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IGSF8/CD316 Antibody [Unconjugated]
EWI2CD81 partner 3
Glu-Trp-Ile EWI motif-containing protein 2
immunoglobulin superfamily, member 8
Keratinocytes-associated transmembrane protein 4
PGRLimmunoglobulin superfamily member 8
IGSF8, also known as PGRL (PG Regulatory-Like Protein), KASP (KAI/CD82 Associated Protein) and EWI-2 (Glu-Trp-Ile motif 2), is a widely expressed transmembrane adhesion protein. It interacts with beta 1 Integrins and various tetraspanins including CD9, CD81 and CD82. IGSF8 contains four extracellular Ig-like domains. IGSF8 over-expression in transformed cells inhibits cell migration and suppresses cancer metastatic potential. Mouse and human IGSF8 share 90% amino acid sequence identity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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