IGSF8/CD316 Antibody [Unconjugated]

Images

 
Neuro‑2A mouse neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117, filled histogram) or isotype control antibody (Catalog # ...read more
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, DU145 human prostate carcinoma cell line, and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
Western blot shows lysates of rat brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) ...read more
Simple Western lane view shows lysates of human cerebellum tissue, human hippocampus tissue, Neuro‑2A mouse neuroblastoma cell line, and rat hippocampus tissue, loaded at 0.2 mg/mL. A specific band was ...read more
IGSF8/CD316 was detected in immersion fixed Neuro‑2A mouse neuroblastoma cell line using Goat Anti-Human/Mouse/Rat IGSF8/CD316 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3117) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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IGSF8/CD316 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGSF8/CD316
Ala25-Thr577
Accession # NP_536344
Specificity
Detects mouse IGSF8/CD316 in direct ELISAs. Detects human, mouse, and rat IGSF8/CD316 in Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGSF8 is observed and less than 1% cross-reactivity with recombinant mouse IGSF4 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGSF8
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 20 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF3117 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGSF8/CD316 Antibody [Unconjugated]

  • CD316 antigen
  • CD316
  • CD81P3
  • CD81P3LIR-D1
  • EWI2
  • EWI-2
  • EWI2CD81 partner 3
  • Glu-Trp-Ile EWI motif-containing protein 2
  • IGSF8
  • immunoglobulin superfamily, member 8
  • KCT4
  • KCT-4
  • Keratinocytes-associated transmembrane protein 4
  • PGRL
  • PGRLimmunoglobulin superfamily member 8

Background

IGSF8, also known as PGRL (PG Regulatory-Like Protein), KASP (KAI/CD82 Associated Protein) and EWI-2 (Glu-Trp-Ile motif 2), is a widely expressed transmembrane adhesion protein. It interacts with beta 1 Integrins and various tetraspanins including CD9, CD81 and CD82. IGSF8 contains four extracellular Ig-like domains. IGSF8 over-expression in transformed cells inhibits cell migration and suppresses cancer metastatic potential. Mouse and human IGSF8 share 90% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGSF8/CD316 Antibody (AF3117)(3)

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF3117

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Bioinformatics

Gene Symbol IGSF8
Uniprot