TSPAN2 Antibody (822509)

NS0 mouse myeloma cell line co-transfected with human TSPAN2 and eGFP was stained with either (A) Mouse IgG2B Isotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human TSPAN2 Monoclonal Antibody (Catalog # MAB7876) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 822509
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TSPAN2 Antibody (822509) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human TSPAN2
  Met1-Ile221
  Accession # O60636
• Specificity: Detects human TSPAN2 in ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSPAN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN2 Antibody (822509)

• FLJ12082
• NET3
• new EST tetraspan 3
• tetraspan 2
• Tetraspan NET-3
• tetraspan TM4SF
• tetraspanin 2 isoform
• tetraspanin 2
• tetraspanin-2
• TSN2
• TSPAN2
• TSPAN-2

Background

TSPAN2 (tetraspanin-2) is a member of the transmembrane 4 superfamily. It is a cell surface 221 amino acid (aa) protein with 4 transmembrane segments and a large extracellular loop (aa 112‑188) that contains four conserved cysteines and one potential N-glycosylation site. Both the N- and C-termini are intracellular. In humans, TSPAN2 expressed sequence tags have been found in the pregnant uterus, T cell, and fetal heart. In rats, TSPAN2 is thought to be involved in oligodendrocyte differentiation. Human TSPAN2 shares 94% and 95% aa sequence identity with mouse and rat TSPAN2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSPAN2
• Uniprot:
  • O60636()