Genetic Strategies: Knockdown Validated: Stathmin-2/STMN2 Antibody [NBP1-49461] - Analysis of endogenous STMN2 in mouse dorsal root ganglia (DRG) neurons. A partial siRNA knockdown was used in the second lane. ...read more
Immunocytochemistry/ Immunofluorescence: Stathmin-2/STMN2 Antibody [NBP1-49461] - Staining of STMN2 in primary mouse dorsal root ganglia (DRG) neurons Image shows the expected staining of endogenous STMN2 in axons and ...read more
Immunohistochemistry-Frozen: Stathmin-2/STMN2 Antibody [NBP1-49461] - Neuronal deletion of Tsc2 improves axon regeneration within the first 2 d following nerve injury. SCG10 immunostaining of sciatic nerves 1 d after ...read more
Expression of Tagged Proteins: Immunocytochemistry/ Immunofluorescence: Stathmin-2/STMN2 Antibody [NBP1-49461] - Staining of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used ...read more
Expression of Tagged Proteins: Western Blot: Stathmin-2/STMN2 Antibody [NBP1-49461] - Analysis of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used at a dilution of 1:4000. ...read more
Immunohistochemistry-Frozen: Stathmin-2/STMN2 Antibody [NBP1-49461] - Stathmin-2/STMN2 immunoreactivity at the trigeminal root entry zone (antibody diluted 1:1000, tissue taken from a mouse with experimental autoimmune ...read more
Hu, Mu, Rt, BvSpecies Glossary
WB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, In vivo, KD
In Western blot a band can be seen at ~22 kDa. Use for in vivo assays reported in the scientific literature (PMID: 22726832). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 31182472). Use In vitro assay reported in scientific literature (PMID: 22726832). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 4.5 using NBP1-49461 in the following applications:
Mouse reactivity reported in scientific literature (PMID:32778834).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Alternate Names for Stathmin-2/STMN2 Antibody
neuron-specific growth-associated protein
SCG10neuronal growth-associated protein (silencer element)
superior cervical ganglia, neural specific 10
Superior cervical ganglion-10 protein
STMN2 (Stathmin-2 or SCG10/Scgn10) belongs to stathmin family of proteins which are known to bind tubulin for acting as a sequestering agents and for promoting microtubule disassembly. STMN2 is a membrane-associated neuronal protein expressed mainly during development and its expression correlates with neurite outgrowth. STMN2 possesses a unique N-terminal domain that is critical for membrane binding, is responsible for STMN2 localization to the Golgi complex and is important in its targeting to growth cones. STMN2 localize to perinuclear cytoplasm, axons, and growth cones of developing neurons, and exists in soluble as well as membrane-bound forms. STMN2 interacts with ITMC2, as well as MAPK8, and when phosphorylated by MAPK8, it act as regulator of microtubule stability which controls neurite length in cortical neurons. Unlike other stathmins, STMN2 acts in two ways to promote microtubule dynamics: it stabilizes microtubules at their plus end and promotes microtubule catastrophe at their minus end. In the developing brain, STMN2 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone. Reduced STMN2 expression is associated with Down's syndrome and Alzheimer's disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Giovanni S, serger e, Chadwick J et al. The intermittent fasting-dependent gut microbial metabolite indole-3 propionate promotes nerve regeneration and recovery after injury Research Square Dec 16 2020 (IHC, Mouse)
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