Stathmin-2/STMN2 Antibody

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Genetic Strategies: Knockdown Validated: Stathmin-2/STMN2 Antibody [NBP1-49461] - Analysis of endogenous STMN2 in mouse dorsal root ganglia (DRG) neurons. A partial siRNA knockdown was used in the second lane. ...read more
Immunocytochemistry/ Immunofluorescence: Stathmin-2/STMN2 Antibody [NBP1-49461] - Staining of STMN2 in primary mouse dorsal root ganglia (DRG) neurons Image shows the expected staining of endogenous STMN2 in axons and ...read more
Immunohistochemistry-Frozen: Stathmin-2/STMN2 Antibody [NBP1-49461] - Neuronal deletion of Tsc2 improves axon regeneration within the first 2 d following nerve injury. SCG10 immunostaining of sciatic nerves 1 d after ...read more
Expression of Tagged Proteins: Immunocytochemistry/ Immunofluorescence: Stathmin-2/STMN2 Antibody [NBP1-49461] - Staining of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used ...read more
Expression of Tagged Proteins: Western Blot: Stathmin-2/STMN2 Antibody [NBP1-49461] - Analysis of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used at a dilution of 1:4000. ...read more
Immunohistochemistry-Frozen: Stathmin-2/STMN2 Antibody [NBP1-49461] - Stathmin-2/STMN2 immunoreactivity at the trigeminal root entry zone (antibody diluted 1:1000, tissue taken from a mouse with experimental autoimmune ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, In vivo, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Stathmin-2/STMN2 Antibody Summary

Immunogen
C-terminal peptide of mouse STMN2. [Swiss-Prot P55821]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STMN2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:4000
  • Immunocytochemistry/Immunofluorescence 1:4000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin
  • In vitro assay
  • In vivo assay
  • Knockdown Validated
Application Notes
In Western blot a band can be seen at ~22 kDa. Use for in vivo assays reported in the scientific literature (PMID: 22726832). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 31182472). Use In vitro assay reported in scientific literature (PMID: 22726832). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-49461 in the following applications:

Publications
Read Publications using
NBP1-49461 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32778834).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Stathmin-2/STMN2 Antibody

  • FLJ34868
  • FLJ50995
  • neuron-specific growth-associated protein
  • Protein SCG10
  • SCG10
  • SCG10neuronal growth-associated protein (silencer element)
  • SCGN10
  • SCGN10stathmin-2
  • Stathmin2
  • Stathmin-2
  • stathmin-like 2
  • STMN2
  • superior cervical ganglia, neural specific 10
  • Superior cervical ganglion-10 protein

Background

STMN2 (Stathmin-2 or SCG10/Scgn10) belongs to stathmin family of proteins which are known to bind tubulin for acting as a sequestering agents and for promoting microtubule disassembly. STMN2 is a membrane-associated neuronal protein expressed mainly during development and its expression correlates with neurite outgrowth. STMN2 possesses a unique N-terminal domain that is critical for membrane binding, is responsible for STMN2 localization to the Golgi complex and is important in its targeting to growth cones. STMN2 localize to perinuclear cytoplasm, axons, and growth cones of developing neurons, and exists in soluble as well as membrane-bound forms. STMN2 interacts with ITMC2, as well as MAPK8, and when phosphorylated by MAPK8, it act as regulator of microtubule stability which controls neurite length in cortical neurons. Unlike other stathmins, STMN2 acts in two ways to promote microtubule dynamics: it stabilizes microtubules at their plus end and promotes microtubule catastrophe at their minus end. In the developing brain, STMN2 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone. Reduced STMN2 expression is associated with Down's syndrome and Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Stathmin-2/STMN2 Antibody (NBP1-49461)(37)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, In vivo, WB.


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ICC/IF
(11)
IHC
(15)
IHC-Fr
(1)
IHC-P
(1)
In vivo
(2)
WB
(3)
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Human
(3)
Mouse
(20)
Rat
(6)
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Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NBP1-49461 Applications Species
Chen Q, Liu Q, Zhang Y et al. Leukemia inhibitory factor regulates Schwann cell proliferation and migration and affects peripheral nerve regeneration Cell death & disease Apr 22 2021 [PMID: 33888681] (In vivo, Rat) In vivo Rat
Wang Y, Zhang F, Zhang Y et al. Betacellulin Regulates Peripheral Nerve Regeneration by Affecting Schwann Cell Migration and Axon Elongation Research Square Feb 5 2021 [PMID: 33794764] (IHC, Rat) IHC Rat
Nadeau JR, Arnold BM, Johnston JM et al. Acute intermittent hypoxia enhances regeneration of surgically repaired peripheral nerves in a manner akin to electrical stimulation Experimental neurology Mar 5 2021 [PMID: 33684407] (IHC, Rat) IHC Rat
Kim H, Lee J, Cho Y PDK1 is a negative regulator of axon regeneration Molecular brain Feb 12 2021 [PMID: 33579325] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Kalinski AL, Yoon C, Huffman LD et al. Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement eLife Dec 2 2020 [PMID: 33263277] (ICC/IF, Mouse) ICC/IF Mouse
Franco A, Dang X, Walton EK et al. Burst mitofusin activation reverses neuromuscular dysfunction in murine CMT2A Elife Oct 19 2020 [PMID: 33074106]
Avraham O, Deng PY, Jones S, Kuruvilla R Fatty acid synthesis in satellite glial cell promotes regenerative growth in sensory neurons bioRxiv (IHC-Fr, Mouse) IHC-Fr Mouse
Jeon Y, Shin JE, Kwon M et al. In Vivo Gene Delivery of STC2 Promotes Axon Regeneration in Sciatic Nerves Mol Neurobiol Oct 4 2020 [PMID: 33011858]
Smaila BD, Holland SD, Babaeijandaghi F et al. Systemic hypoxia mimicry enhances axonal regeneration and functional recovery following peripheral nerve injury Exp. Neurol. Aug 16 2020 [PMID: 32814068] (IHC, Mouse) IHC Mouse
Kong G, Zhou L, Serger E et al. AMPK controls the axonal regenerative ability of dorsal root ganglia sensory neurons after spinal cord injury Nat Metab Aug 10 2020 [PMID: 32778834] (Mouse) Mouse
Show All 37 Publications.

Reviews for Stathmin-2/STMN2 Antibody (NBP1-49461) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using NBP1-49461:
Filter by Applications
IHC-Fr
(2)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Stathmin-2/STMN2 NBP1-49461
Enlarge
5
reviewed by:
Kevin Thorburn
IHC-Fr Mouse 11/29/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse trigeminal root entry zone,trigeminal root entry zone
SpeciesMouse
LotC-7
  4
reviewed by:
Verified Customer
IHC-Fr Other 08/31/2016
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedsciatic nerve
SpeciesOther
Lotc1

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Recent Reviews

4.5
5
1
4
1
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0
2
0
1
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Kevin Thorburn
11/29/2018
Application: IHC-Fr
Species: Mouse

 
Verified Customer
08/31/2016
Application: IHC-Fr
Species: Other

Bioinformatics

Gene Symbol STMN2