Stathmin-2/STMN2 Antibody

Images

 
Genetic Strategies: Western Blot: Stathmin-2/STMN2 Antibody [NBP1-49461] - Analysis of endogenous STMN2 in mouse dorsal root ganglia (DRG) neurons. A partial siRNA knockdown was used in the second lane. ...read more
Immunocytochemistry/ Immunofluorescence: Stathmin-2/STMN2 Antibody [NBP1-49461] - Staining of STMN2 in primary mouse dorsal root ganglia (DRG) neurons Image shows the expected staining of endogenous STMN2 in axons and ...read more
Immunohistochemistry-Frozen: Stathmin-2/STMN2 Antibody [NBP1-49461] - Stathmin-2/STMN2 immunoreactivity at the trigeminal root entry zone (antibody diluted 1:1000, tissue taken from a mouse with experimental autoimmune ...read more
Western Blot: Stathmin-2/STMN2 Antibody [NBP1-49461] - Analysis of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used at a dilution of 1:4000. Image courtesy of Dr. Jung Eun Shin.
Immunocytochemistry/ Immunofluorescence: Stathmin-2/STMN2 Antibody [NBP1-49461] - Staining of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used at a dilution of 1:1000 (A, B) and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, In vivo
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Stathmin-2/STMN2 Antibody Summary

Immunogen
C-terminal peptide of mouse STMN2. [Swiss-Prot P55821]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STMN2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:4000
  • Immunocytochemistry/Immunofluorescence 1:4000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • In vivo assay
Application Notes
In Western blot a band can be seen at ~22 kDa. Use for in vivo assays reported in the scientific literature (PMID: 22726832). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49461 in the following applications:

Publications
Read Publications using
NBP1-49461 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Stathmin-2/STMN2 Antibody

  • FLJ34868
  • FLJ50995
  • neuron-specific growth-associated protein
  • Protein SCG10
  • SCG10
  • SCG10neuronal growth-associated protein (silencer element)
  • SCGN10
  • SCGN10stathmin-2
  • Stathmin2
  • Stathmin-2
  • stathmin-like 2
  • STMN2
  • superior cervical ganglia, neural specific 10
  • Superior cervical ganglion-10 protein

Background

STMN2 (Stathmin-2 or SCG10/Scgn10) belongs to stathmin family of proteins which are known to bind tubulin for acting as a sequestering agents and for promoting microtubule disassembly. STMN2 is a membrane-associated neuronal protein expressed mainly during development and its expression correlates with neurite outgrowth. STMN2 possesses a unique N-terminal domain that is critical for membrane binding, is responsible for STMN2 localization to the Golgi complex and is important in its targeting to growth cones. STMN2 localize to perinuclear cytoplasm, axons, and growth cones of developing neurons, and exists in soluble as well as membrane-bound forms. STMN2 interacts with ITMC2, as well as MAPK8, and when phosphorylated by MAPK8, it act as regulator of microtubule stability which controls neurite length in cortical neurons. Unlike other stathmins, STMN2 acts in two ways to promote microtubule dynamics: it stabilizes microtubules at their plus end and promotes microtubule catastrophe at their minus end. In the developing brain, STMN2 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone. Reduced STMN2 expression is associated with Down's syndrome and Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Stathmin-2/STMN2 Antibody (NBP1-49461)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, In vivo, WB.


Filter By Application
ICC/IF
(9)
IHC
(4)
IHC-P
(1)
In vivo
(1)
WB
(2)
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Human
(3)
Mouse
(10)
Rat
(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP1-49461 Applications Species
Holland, SD;Ramer, LM;McMahon, SB;Denk, F;Ramer, MS; An ATF3-CreERT2 Knock-In Mouse for Axotomy-Induced Genetic Editing: Proof of Principle eNeuro Apr 9 2019 [PMID: 30993183] (ICC/IF, Mouse) ICC/IF Mouse
Okada M. A Novel Phosphorylation site of GAP-43 is a Good Molecular Marker for Axon Regeneration after Nerve Injury. Thesis. Mar 23 2016 (IHC, Mouse) IHC Mouse
Carlin D, Halevi AE, Ewan EE et al. Nociceptor Deletion of Tsc2 Enhances Axon Regeneration by Inducing a Conditioning Injury Response in Dorsal Root Ganglia eNeuro Jun 25 2019 [PMID: 31182472] (IHC-P, Mouse) IHC-P Mouse
Hutson TH, Kathe C, Palmisano I et al. Cbp-dependent histone acetylation mediates axon regeneration induced by environmental enrichment in rodent spinal cord injury models Sci Transl Med Apr 10 2019 [PMID: 30971452]
Davies AJ, Kim HW, Gonzalez-Cano R et al. Natural Killer Cells Degenerate Intact Sensory Afferents following Nerve Injury Cell Feb 7 2019 [PMID: 30712871] (ICC/IF, Mouse) ICC/IF Mouse
Melamed Z, Lopez-Erauskin J, Baughn MW et al. Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegeneration. Nat. Neurosci. Feb 1 2019 [PMID: 30643298] (WB, Human) WB Human
Klim JR, Williams LA, Limone F et al. ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair. Nat. Neurosci. Feb 1 2019 [PMID: 30643292] (ICC/IF, WB, IHC, Human) ICC/IF, WB, IHC Human
Frey E, Karney-Grobe S, Krolak T et al. TRPV1 Agonist, Capsaicin, Induces Axon Outgrowth after Injury via Ca2+/PKA Signaling eNeuro May 30 2018 [PMID: 29854941] (ICC/IF, Mouse) ICC/IF Mouse
Cao Y, Wang H, Zeng W. Whole-tissue 3D imaging reveals intra-adipose sympathetic plasticity regulated by NGF-TrkA signal in cold-induced beiging. Protein Cell. Mar 27 2018 [PMID: 29589323] (ICC/IF, Mouse) ICC/IF Mouse
Weng YL, Wang X, An R et al. Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System Neuron 2018 Jan 17 [PMID: 29346752] (Mouse) Mouse
Show All 19 Publications.

Review for Stathmin-2/STMN2 Antibody (NBP1-49461) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-49461:
Filter by Applications
IHC-Fr
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Stathmin-2/STMN2 NBP1-49461
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reviewed by:
Kevin Thorburn
IHC-Fr Mouse 11/29/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse trigeminal root entry zone,trigeminal root entry zone
SpeciesMouse
LotC-7

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Recent Reviews

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Kevin Thorburn
11/29/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol STMN2