Stathmin-2/STMN2 Antibody

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Western Blot: Stathmin-2/STMN2 Antibody [NBP1-49461] - Analysis of endogenous STMN2 in mouse dorsal root ganglia (DRG) neurons. A partial siRNA knockdown was used in the second lane. NBP1-49461 was used at a dilution ...read more
Immunocytochemistry/ Immunofluorescence: Stathmin-2/STMN2 Antibody [NBP1-49461] - Staining of STMN2 in primary mouse dorsal root ganglia (DRG) neurons. Shows the expected staining of endogenous STMN2 in axons and ...read more
Western Blot: Stathmin-2/STMN2 Antibody [NBP1-49461] - Analysis of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used at a dilution of 1:4000. Image courtesy of Dr. Jung Eun Shin.
Immunocytochemistry/ Immunofluorescence: Stathmin-2/STMN2 Antibody [NBP1-49461] - Staining of STMN2 in HEK293T cells transfected with GFP or a GFP-STMN2 fusion. NBP1-49461 was used at a dilution of 1:1000 (A, B) and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, In vivo
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
     

Genetic Strategies

   

Order Details

Stathmin-2/STMN2 Antibody Summary

Immunogen
C-terminal peptide of mouse STMN2. [Swiss-Prot P55821]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STMN2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:4000
  • Immunocytochemistry/Immunofluorescence 1:4000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • In vivo assay
Application Notes
This STMN2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western blot, where a band can be seen at ~22 kDa. Use for in vivo assays reported in the scientific literature (PMID: 22726832). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-49461 in the following applications:

Publications
Read Publications using
NBP1-49461 in the following applications:

Reactivity Notes

Human, mouse, rat and bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Stathmin-2/STMN2 Antibody

  • FLJ34868
  • FLJ50995
  • neuron-specific growth-associated protein
  • Protein SCG10
  • SCG10
  • SCG10neuronal growth-associated protein (silencer element)
  • SCGN10
  • SCGN10stathmin-2
  • Stathmin2
  • Stathmin-2
  • stathmin-like 2
  • STMN2
  • superior cervical ganglia, neural specific 10
  • Superior cervical ganglion-10 protein

Background

STMN2 (Stathmin-2 or SCG10/Scgn10) belongs to stathmin family of proteins which are known to bind tubulin for acting as a sequestering agents and for promoting microtubule disassembly. STMN2 is a membrane-associated neuronal protein expressed mainly during development and its expression correlates with neurite outgrowth. STMN2 possesses a unique N-terminal domain that is critical for membrane binding, is responsible for STMN2 localization to the Golgi complex and is important in its targeting to growth cones. STMN2 localize to perinuclear cytoplasm, axons, and growth cones of developing neurons, and exists in soluble as well as membrane-bound forms. STMN2 interacts with ITMC2, as well as MAPK8, and when phosphorylated by MAPK8, it act as regulator of microtubule stability which controls neurite length in cortical neurons. Unlike other stathmins, STMN2 acts in two ways to promote microtubule dynamics: it stabilizes microtubules at their plus end and promotes microtubule catastrophe at their minus end. In the developing brain, STMN2 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone. Reduced STMN2 expression is associated with Down's syndrome and Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Stathmin-2/STMN2 Antibody (NBP1-49461)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, In vivo.


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ICC/IF
(3)
IHC
(3)
In vivo
(1)
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Human
(1)
Mouse
(4)
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Showing Publications 1 - 9 of 9.
Publications using NBP1-49461 Applications Species
Weng YL, An R, Cassin J et al. An Intrinsic Epigenetic Barrier for Functional Axon Regeneration. Neuron. Apr 19 2017 [PMID: 28426967]
Rouger V, Alchini R, Kazarine A et al. Low-cost multimodal light sheet microscopy for optically cleared tissues and living specimens. J Biomed Opt. Dec 1 2016 [PMID: 27999866] (IHC, Human) IHC Human
Chen L, Liu Z, Zhou B et al. CELF RNA binding proteins promote axon regeneration in C. elegans and mammals through alternative splicing of Syntaxins. Elife 2016 Jun 02 [PMID: 27253061]
Valakh V, Frey E, Babetto E et al. Cytoskeletal disruption activates the DLK/JNK pathway, which promotes axonal regeneration and mimics a preconditioning injury Neurobiol. Dis. 2015 Feb 26 [PMID: 25726747] (ICC/IF, Mouse)

Details:
Stathmin-2/STMN2 antibody used for ICC-IF staiing on murine embryonic spot cultures and dissociated adult dorsal root ganglia / DRG neurons cultures. The assay involved - 4% paraformaldehyde-PBS fixation for 20 minutes at RT, permeablization with PBST, use of primary antibody at 1:5000 dilution with ON 4C incubation, detection using fluorochrome conjugated secondary antibody.
ICC/IF Mouse
Gobrecht P, Leibinger M, Andreadaki A, Fischer D. Sustained GSK3 activity markedly facilitates nerve regeneration. Nat Commun. 2014 Aug 01 [PMID: 25078444]
Shin Jung Eun, Geisler Stefanie, DiAntonio Aaron. Dynamic regulation of SCG10 in regenerating axons after injury. Exp Neurol. 2014 Feb 01 [PMID: 24246279]
Cho Y, Sloutsky R, Naegle KM, Cavalli V. Injury-Induced HDAC5 Nuclear Export Is Essential for Axon Regeneration. Cell. 2013 Nov 7 [PMID: 24209626] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Shin JE, Cho Y, Beirowski B, Milbrandt J et al. Dual leucine zipper kinase is required for retrograde injury signaling and axonal regeneration Neuron 2012 Jun 21 [PMID: 22726832] (ICC/IF, In vivo, Mouse) ICC/IF, In vivo Mouse
Okada M. A Novel Phosphorylation site of GAP-43 is a Good Molecular Marker for Axon Regeneration after Nerve Injury. Thesis. Mar 23 2016 (IHC, Mouse) IHC Mouse

Review for Stathmin-2/STMN2 Antibody (NBP1-49461) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NBP1-49461:
Filter by Applications
IHC-Fr
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Stathmin-2/STMN2 NBP1-49461
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reviewed by:
Anonymous
IHC-Fr Mouse 08/31/2016
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedsciatic nerve
SpeciesMouse
Lotc1

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Recent Reviews

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Anonymous
08/31/2016
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol STMN2
Entrez