Recombinant Human TSLP Protein

SDS-Page: Human TSLP Protein [NBP2-35083]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human TSLP Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 132 amino acids corresponding to TSLP Source: E. coli

  Uniprot ID: Q969D9

  Amino Acid Sequence: MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ

• Details of Functionality: TSLP protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-7R alpha and human TSLP R co-transfected murine BaF3 pro-B cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 x 10^6 IU/mg
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TSLP
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of TSLP as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 15.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human TSLP Protein

• thymic stromal lymphopoietin
• TSLP

Background

Thymic stromal lymphopoietin (TSLP) is a hemopoietic protein belonging to the cytokine family and is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. It is mainly expressed in a number of tissues including heart, liver and prostate. TSLP signals through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. After binding STAT5 phosphorylation is induced resulting in the expression of downstream transcription factors. Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP has the functions of enhancing the maturation of CD11c+ dendritic cells and inducing allergic inflammation by directly activating mast cells. It expression is linked to many disease states including asthma, inflammatory arthritis, atopic dermatitis, and eczema and other allergic states. But the factors inducing the activation of TSLP release are not clearly defined. Human TSLP shares approximately 43 % a.a. sequence identity with mouse TSLP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for TSLP Recombinant Protein (NBP2-35083)(2)

Publications using NBP2-35083

• Luisa Canè, Remo Poto, Francesco Palestra, Ilaria Iacobucci, Marinella Pirozzi, Seetharaman Parashuraman, Anne Lise Ferrara, Amalia Illiano, Antonello La Rocca, Edoardo Mercadante, Piero Pucci, Gianni Marone, Giuseppe Spadaro, Stefania Loffredo, Maria Monti, Gilda Varricchi, Anagha Malur Thymic Stromal Lymphopoietin (TSLP) Is Cleaved by Human Mast Cell Tryptase and Chymase International Journal of Molecular Sciences 2024-04-05 [PMID: 38612858]
• Marcella S, Petraroli A, Canè L et al. Thymic stromal lymphopoietin (TSLP) is a substrate for tryptase in patients with mastocytosis European Journal of Internal Medicine 2023-07-26 [PMID: 37500310]

Bioinformatics

• Gene Symbol: TSLP
• Uniprot:
  • Q969D9()
  • Q969D9()
  • Q969D9()