TSG101 Antibody

Images

 
Western Blot: TSG101 Antibody [NBP2-20748] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody.
Immunocytochemistry/ Immunofluorescence: TSG101 Antibody [NBP2-20748] - A549 cells were fixed in 2% paraformaldehyde at RT for 15 min, and be permeabilized in 0.1% saponin, 10% NGS, 100mM glycine for 30 min. Green: ...read more
Western Blot: TSG101 Antibody [NBP2-20748] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: TSG101 Antibody [NBP2-20748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody diluted at 1:500. The signal was developed with Trident ...read more
Western Blot: TSG101 Antibody [NBP2-20748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody diluted at 1:1000.
Western Blot: TSG101 Antibody [NBP2-20748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: TSG101 Antibody [NBP2-20748] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using TSG101 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled ...read more
Immunoprecipitation: TSG101 Antibody [NBP2-20748] - Immunoprecipitation of TSG101 protein from A549 whole cell extracts using 5 ug of TSG101 antibody. Western blot analysis was performed using TSG101 antibody. EasyBlot ...read more
ELISA: TSG101 Antibody [NBP2-20748] - ELISA detection of TSG101 using for capture at a concentration of 5 ug/mL and for detection at a concentration of 1.5 ug/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Order Details

TSG101 Antibody Summary

Immunogen
The exact sequence is proprietary.
Localization
Cytoplasm; Membrane; Peripheral membrane protein; Nucleus; Late endosome membrane; Peripheral membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TSG101
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TSG101 Antibody

  • ESCRT-I complex subunit TSG101
  • TSG10
  • tumor susceptibility gene 10
  • tumor susceptibility gene 101 protein
  • tumor susceptibility gene 101
  • tumor susceptibility protein
  • VPS23

Background

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TSG101 Antibody (NBP2-20748)

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Blogs on TSG101.

Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

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Bioinformatics

Gene Symbol TSG101