TSC22/TSC22D1 Antibody (OTI4H8)

Western Blot: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D1.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Human tonsil tissue.

Flow Cytometry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Jurkat cells, using TSC22D1 antibody,(Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of extracts (10ug) from 8 Human tissue by using (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Carcinoma of Human prostate tissue.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Carcinoma of Human liver.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Human pancreas tissue.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Human liver tissue.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Carcinoma of Human thyroid tissue.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Adenocarcinoma of Human colon tissue.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Human Kidney tissue.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Human breast tissue.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Human prostate tissue.

Immunohistochemistry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Carcinoma of Human kidney tissue.

Flow Cytometry: TSC22/TSC22D1 Antibody (4H8) [NBP2-46238] - Analysis of Hela cells, using TSC22D1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI4H8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TSC22/TSC22D1 Antibody (OTI4H8) Summary

• Immunogen: Full length human recombinant protein of human TSC22D1(NP_006013) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSC22D1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 15.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TSC22/TSC22D1 Antibody (OTI4H8)

• Cerebral protein 2
• Egr5
• KIAA1994
• MGC17597
• Regulatory protein TSC-22
• RP11-269C23.2
• TGFB1I4
• TGFB-stimulated clone 22 homolog
• transforming growth factor beta 1 induced transcript 4
• Transforming growth factor beta-1-induced transcript 4 protein
• transforming growth factor beta-stimulated protein TSC-22
• TSC22 domain family 1
• TSC22 domain family protein 1
• TSC22 domain family, member 1
• TSC22
• TSC22D1
• TSC22DKFZp686O19206

Background

Transforming growth factor-beta-stimulated clone-22 (TSC-22) acts as a transcriptionalregulator to modulate cell growth and differentiation and cell death. TSC-22 contains a leucine zipper domain as well as a nuclear export signal, resulting in cytoplasmic localization in living cells. However, concomitant with the induction of apoptosis, TSC-22 translocates from the cytoplasm to the nucleus and shows transcriptional regulatory activity. TSC-22 acts as a major downstream component in the TGF-beta pathway, and also the PPARgamma signalling pathway. The association of these two pathways with tumor suppression, and the significant downregulation of TSC-22 mRNA in various cancer types, such as brain and salivary gland tumors, imply an antiproliferative role for TSC-22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSC22/TSC22D1 Antibody (NBP2-46238)(2)

Publications using NBP2-46238

• Zheng L, Ling W, Zhu D et al. Roquin-1 resolves sepsis-associated acute liver injury by regulating inflammatory profiles via miRNA cargo in extracellular vesicles iScience 2023-08-01 [PMID: 37554446] (ICC/IF, Mouse)
• Liu X, Geng Y, Liang J et al. HER2 drives lung fibrosis by activating a metastatic cancer signature in invasive lung fibroblasts The Journal of experimental medicine 2022-10-03 [PMID: 35980387] (WB, Human)

Bioinformatics

• Gene Symbol: TSC22D1