TSC1 Antibody

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Western Blot: TSC1 Antibody [NB100-2315] - Detection of Human and Mouse TSC1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity ...read more
Western Blot: TSC1 Antibody [NB100-2315] - Detection of Human and Mouse TSC1 on HeLa whole cell lysate using NB100-2315.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TSC1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1164) of human tuberous sclerosis 1 using the numbering given in Swiss-Prot entry Q92574 (GeneID 7248).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TSC1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:25000
  • Immunoprecipitation 1-5 ug/mg lysate
Control
TSC1 Overexpression Lysate
Publications
Read Publications using NB100-2315.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TSC1 Antibody

  • Hamartin
  • KIAA0243MGC86987
  • LAMhamartin
  • TSC
  • TSC1
  • Tuberous sclerosis 1 protein
  • tuberous sclerosis 1
  • tumor suppressor

Background

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle progression through its ability to integrate signals from nutrient levels and growth factors. TOR regulation is accomplished through a network of various activators and repressors. It is phosphorylated by Akt, whose activity is indirectly inhibited by the lipid phosphatase PTEN. TOR is normally associated with the regulatory proteins RAPTOR, a scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation, and GBetaL, which stimulates TOR's kinase activity towards downstream proteins. It is further regulated by the proteins Rheb, TSC1 and TSC2, which act to modulate TOR activity. The downstream targets of TOR are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs) whose activation leads to increased protein translation and cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TSC1 Antibody (NB100-2315)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for TSC1 Antibody (NB100-2315)

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Blogs on TSC1.

TSC1 - a negative regulator of mTOR signaling
TSC1 is a tumor suppressor gene that encodes a 130 kDa protein called hamartin. TSC1 was first identified as an oncogenic driver of Tuberous Sclerosis, a condition characterized by numerous benign tumors of the skin, brain, heart, and lungs. A mut...  Read full blog post.

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Calculators

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Bioinformatics

Gene Symbol TSC1
Entrez
Uniprot