Tryptophan Hydroxylase 1/TPH-1 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

Tryptophan Hydroxylase 1/TPH-1 Antibody [Alexa Fluor® 350] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tryptophan Hydroxylase 1/TPH‑1
  Met102-Pro403
  Accession # P17752
• Specificity: Detects human Tryptophan Hydroxylase 1/TPH‑1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tryptophan Hydroxylase 1/TPH-1 Antibody [Alexa Fluor® 350]

• EC 1.14.16
• EC 1.14.16.4
• indoleacetic acid-5-hydroxylase
• L-tryptophan hydroxylase
• MGC119994
• TPH
• TPH-1
• TPRH
• TRPH
• tryptophan 5-hydroxylase 1
• Tryptophan 5-monooxygenase 1
• tryptophan hydroxylase (tryptophan 5-monooxygenase)
• Tryptophan Hydroxylase 1

Background

Tryptophan Hydroxylase 1 (TPH-1) is a 50‑60 kDa intracellular enzyme that belongs to the biopterin-dependent, aromatic amino acid hydroxylase family. It is expressed in gut enterochromaffin cells and pineal gland. TPH-1 catalyzes the hydroxylation of L-Trp, which is the rate-limiting step in serotonin biosynthesis. Human TPH-1 is 444 amino acids (aa) in length. It contains an N-terminal regulatory domain (aa 1‑98), a catalytic region (aa 99‑424), and a C‑terminal Leu-zipper tetramerization domain (aa 425‑444). TPH-1 is phosphorylated on Ser58, and subsequently binds to 14-3-3 proteins, resulting in activation. It functions as a noncovalent Fe-binding 230 kDa homotetramer. There are three potential splice variants. One shows a 29 aa substitution for aa 438‑444, a second shows a 50 aa substitution for aa 157‑444, and a third shows a 68 aa substitution for aa 1‑21. Over aa 102‑403, Human TPH-1 shares 95% aa identical with mouse TPH-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.