Trypsin Pan Specific/PRSS1/2/3 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Trypsin Pan Specific/PRSS1/2/3 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Trypsin 2 (R&D Systems, Catalog # 3586-SE)
  Ala16-Ser247
  Accession # P07478
• Specificity: Detects human Trypsin 1, human Trypsin 2 and human Trypsin 3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trypsin Pan Specific/PRSS1/2/3 Antibody [Biotin]

• TRY2
• TRY8
• TRYP2
• Trypsin

Background

Trypsin is a general term for any of three 24 kDa gene products that belong to the peptidase S1 family of enzymes.  Trypsin in Greek means “rubbing or friction”, and it was chosen here because the first trypsins were extracted from pancreas via a glycerin-based rubbing maceration.  Trypsin-1 (cationic), -2 (anionic) , and -3 (mesotrypsin) are synthesized as 26 kDa trypsinogens (plus a 35 kDa trypsinogen-3 isoform) that are 247 amino acids (aa) in length.  The first 15 aa constitute a signal sequence, followed by an enterokinase-cleavable eight aa propeptide, and a 224 aa mature molecule.  Asp194 is linked to enzyme activity, and Tyr154 is sulfated.  Over their mature regions, the three trypsins share 84% aa identity.  Mouse trypsin-1 shares 74% aa identity with the human trypsin consensus sequence.  Trypsin-1 and -2 cleave peptide bonds carboxylterminal to a Lys or Arg.  Trypsin-3 has limited activity on trypsin inhibitors, and thus regulates general trypsin activity.  Trypsin-1, -2, and -3 are expressed by multiple tissues, including pancreas (for general digestion), liver, lung and skin.  In blood, trypsinogen-1 and -2 circulate at ng/mL concentrations.  In pancreatic fluid, trypsin-1, -2, and -3 are found in a 58:40:2 ratio.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Trypsin Pan Specific/PRSS1/2/3 Antibody (BAF3586)(1)

Publication using BAF3586

• Lindau-Shepard BA, Pass KA Newborn screening for cystic fibrosis by use of a multiplex immunoassay. Clin. Chem., 2009-12-29;56(3):445-50. 2009-12-29 [PMID: 20040620] (Immunoassay Development, Human)

Bioinformatics

• Uniprot:
  • P07478()