Trypsin 1/PRSS1 Antibody [Alexa Fluor® 350]

• Applications: WB, IHC, IP, B/N
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Trypsin 1/PRSS1 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Trypsin 2
  Ala16-Ser247
  Accession # P07478
• Specificity: Detects human Trypsin Pan Specific (PRSS1/2/3) in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Trypsin is a general term for any of three 24 kDa gene products that belong to the peptidase S1 family of enzymes. Trypsin in Greek means “rubbing or friction”, and it was chosen here because the first trypsins were extracted from pancreas via a glycerin-based rubbing maceration. Trypsin-1 (cationic), -2 (anionic) , and -3 (mesotrypsin) are synthesized as 26 kDa trypsinogens (plus a 35 kDa trypsinogen-3 isoform) that are 247 amino acids (aa) in length. The first 15 aa constitute a signal sequence, followed by an enterokinase-cleavable eight aa propeptide, and a 224 aa mature molecule. Asp194 is linked to enzyme activity, and Tyr154 is sulfated. Over their mature regions, the three trypsins share 84% aa identity.  Mouse trypsin-1 shares 74% aa identity with the human trypsin consensus sequence. Trypsin-1 and -2 cleave peptide bonds carboxylterminal to a Lys or Arg. Trypsin-3 has limited activity on trypsin inhibitors, and thus regulates general trypsin activity. Trypsin-1, -2, and -3 are expressed by multiple tissues, including pancreas (for general digestion), liver, lung and skin. In blood, trypsinogen-1 and -2 circulate at ng/mL concentrations. In pancreatic fluid, trypsin-1, -2, and -3 are found in a 58:40:2 ratio. This antibody did not neutralize the activity of rhTrypsin 1 and rhTrypsin 3 significantly.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.