Chymotrypsin C/CTRC Antibody

Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Chymotrypsin C/CTRC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6907) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Chymotrypsin C/CTRC at approximately 33-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chymotrypsin C/CTRC was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Chymotrypsin C/CTRC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6907) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Chymotrypsin C/CTRC Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Chymotrypsin C/CTRC
  Cys17-Leu268
  Accession # Q99895
• Specificity: Detects human Chymotrypsin C/CTRC in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CTRC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chymotrypsin C/CTRC Antibody

• Caldecrin
• chymotrypsin C (caldecrin)
• Chymotrypsin C
• chymotrypsin-C
• CLCR
• CLCRserum calcium decreasing factor
• CTRC
• EC 3.4.21
• EC 3.4.21.2
• ELA4
• elastase 4
• Elastase IV

Background

Chymotrypsin C (CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. The CTRC gene encodes a serum calcium-decreasing factor that has chymotrypsin-like protease activity (1). CTRC has broad substrate specificity, but prefers to cleave on the carboxyl side of hydrophobic residues. The enzyme is expressed primarily in the pancreas, and is secreted into the digestive tract. CTRC is a key regulator of activation and degradation of cationic trypsinogen (2, 3). The inappropriate activation of cationic trypsinogen can contribute to the development of pancreatitis (2, 3). CTRC inhibits osteoclast differentiation by suppression of NFATc1 function by a mechanism independent of its proteolytic activity (4).

1. Tomomura, A. et al. (1995) J. Biol. Chem. 270:30315.
2. Szmola, R. and M. Sahin-Toth (2007) Proc. Natl. Acad. Sci. USA 104:11227.
3. Nemoda, Z. and M. Sahin-Toth (2006) J. Biol. Chem. 281:11879.
4. Hasegawa, H. et al. (2010) J. Biol. Chem. 285:25448.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chymotrypsin C/CTRC Antibody (AF6907)(1)

Publication using AF6907

• A Geisz, Z Jancsó, BC Németh, E Hegyi, M Sahin-Tóth Natural single-nucleotide deletion in chymotrypsinogen C gene increases severity of secretagogue-induced pancreatitis in C57BL/6 mice JCI Insight, 2019-06-18;5(0):. 2019-06-18 [PMID: 31211695] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CTRC
• Uniprot:
  • Q99895()