TRPM8 Antibody - BSA Free

Western Blot: TRPM8 Antibody [NB200-145] - Analysis of TRPM8 in transfected COS cells using NB200-145 at 1:750 and incubated overnight at 4C. Secondary rabbit HRP antibody was incubated 1 hour at RT followed by ECL detection.

Immunohistochemistry: TRPM8 Antibody [NB200-145] - Prostate cancer: Formalin-Fixed Paraffin-Embedded (FFPE)

• Reactivity: Hu, Mu, Dr
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRPM8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRPM8 Antibody - BSA Free (NB200-145) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-TRPM8 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptides made to residues 278-292 RNQLEKYISERTIQD and the C-terminus sequence NDLKGLLKEIANKIK of the human trp-p8 protein.
• Specificity: Reacts with residues 278-292 and the C terminus sequence of the human trp-p8 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPM8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500 -1:1000
• Application Notes: This antibody has been tested to work in Western Blot and immunohistochemistry on paraffin and frozen sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 19295141).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRPM8 Antibody - BSA Free

• CMR1
• Long transient receptor potential channel 6
• LTRPC6
• LTrpC-6
• MGC2849
• short form of the TRPM8 cationic channel
• transient receptor potential cation channel, subfamily M, member 8
• Transient receptor potential p8
• transient receptor potential subfamily M member 8
• TRPM8
• TRPP8
• trp-p8
• TRPP8transient receptor potential cation channel subfamily M member 8

Background

A novel gene, trp-p8 has an expression pattern within normal human tissues that is mostly restricted to prostate epithelial cells. Trp-p8 mRNA expression is at moderate levels in normal prostate tissue and appears to be elevated in prostate cancer. Notably, trp-p8 mRNA was also expressed in a number of nonprostatic primary tumors of the breast, colon, lung and skin origin, whereas transcripts encoding trp-p8 were hardly detected or not detected in the corresponding normal human tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPM8 Antibody (NB200-145)(4)

Publications using NB200-145

• Chen LH, Yeh YM, Chen YF et al. Targeting interleukin-20 alleviates paclitaxel-induced peripheral neuropathy Pain 2020-06-01 [PMID: 32068666] (Immunohistochemistry, Insect - Drosophila)
• Xiong S, Wang B, Lin S et al. Activation of Transient Receptor Potential Melastatin Subtype 8 Attenuates Cold-Induced Hypertension Through Ameliorating Vascular Mitochondrial Dysfunction J Am Heart Assoc 2017-08-02 [PMID: 28768647] (WB, Mouse)
• Ma S, Yu H, Zhao Z, Luo Z, Chen J, Ni Y, Jin R, Ma L, Wang P, Zhu Z, Li L, Zhong J, Liu D, Nilius B, Zhu Z. Activation of the cold-sensing TRPM8 channel triggers UCP1-dependent thermogenesis and prevents obesity. J Mol Cell Biol. 2012-02-17 [PMID: 22241835] (ICC/IF, Mouse)
• Peabody NC, Pohl JB, Diao F et al. Characterization of the Decision Network for Wing Expansion in Drosophila Using Targeted Expression of the TRPM8 Channel. J Neurosci;29(11):3343-3353. 2009-01-01 [PMID: 19295141] (IF/IHC, Drosophila)

Bioinformatics

• Gene Symbol: TRPM8
• Entrez:
  • 79054(Human)
• Uniprot:
  • Q7Z2W7(Human)