TRPA1 Antibody

Immunocytochemistry/Immunofluorescence: TRPA1 Antibody [NB100-98841] - IF on vagal nodose ganglia. Image from verified customer review.

• Reactivity: Mu, Rt, Gp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

TRPA1 Antibody Summary

• Description: Novus Biologicals Rabbit TRPA1 Antibody (NB100-98841) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-TRPA1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from rat TRPA1 conjugated to blue carrier protein was used as the antigen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPA1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Application Notes: IF data from customer review.

Reactivity Notes

Guinea pig data from customer review. Mouse reactivity reported in scientific literature (PMID: 27720932).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for TRPA1 Antibody

• ANKTM1
• ANKTM1ankyrin-like with transmembrane domains 1
• Ankyrin-like with transmembrane domains protein 1
• Transformation-sensitive protein p120
• transient receptor potential cation channel subfamily A member 1
• transient receptor potential cation channel, subfamily A, member 1
• TRPA1
• Wasabi receptor

Background

The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. TISSUE SPECIFICITY: Expressed in inner ear. In inner ear, it is found in the distal half of stereocilia, along the length of the kinocilium and at high level in the pericuticular zone (at protein level). Specifically expressed in a subset of nociceptive neurons. Expressed in the same neurons that TRPV1. In contrast, it is not expressed in neurons expressing TRPM8. Expressed in the superior cervical ganglion. DEVELOPMENTAL STAGE: During utricle development, it is first expressed at E15 and E16 and peaks at E17. It drops at E18 but increases again at E19, possibly corresponding to a second wave of hair cells that are generated at E15. Inhibited by ruthenium red, a potent blocker of TRPV channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPA1 Antibody (NB100-98841)(3)

Publications using NB100-98841

• Coavoy-Sanchez SA, Rodrigues L, Teixeira SA et al. Hydrogen sulfide donors alleviate itch secondary to the activation of type-2 protease activated receptors (PAR-2) in mice. Pharmacol. Res. 2016-10-06 [PMID: 27720932] (WB, Mouse)
  
  Details:
  This TRPA1 antibody was used to gauge the effect of TRPA1 antagonist administration on TRPA1 levels in mouse models by comparing to a control group
• Zeng H, Li P, Zhou D et al. Sophocarpine inhibits TRP channels to produce anti-pruritic and analgesic effects in a mouse model of inflammatory itch and pain bioRxiv 2023-10-16 (WB, Mouse)
• Yu S, Gao G, Peterson BZ, Ouyang A. TRPA1 in mast cell activation-induced long-lasting mechanical hypersensitivity of vagal afferent C-fibers in guinea pig esophagus. Am J Physiol Gastrointest Liver Physiol. 2009-07-01 [PMID: 19423751] (ICC/IF, Guinea Pig)

Review for TRPA1 Antibody (NB100-98841) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Guofeng Gao IF Other 09/16/2014

Summary

• Application: Immunofluorescence
• Sample Tested: vagal nodose ganglia
• Species: Other

Bioinformatics

• Gene Symbol: TRPA1
• Entrez:
  • 277328(Mouse)
  • 312896(Rat)